白藜芦醇固体脂质纳米粒抑制小鼠移植性肿瘤H22的研究

Study on the Anti-tumor Effect of Resveratrol-solid Lipid Nanopartieles on Mouse H22 Hepatocellular Carcinoma in vivo

目的:研究白藜芦醇固体脂质纳米粒(resveratrol-solid lipid nanopartieles,Res-SLN)对H22移植性肿瘤模型小鼠的肿瘤抑制作用。方法:将昆明种小鼠接种肝癌H22瘤株造成相应的移植性荷瘤小鼠模型后,随机分为荷瘤模型组、环磷酰胺(CTX)阳性药对照组(25 mg.kg-1),Res-SLN低、中、高(35,70,105 mg.kg-1)3个剂量组、白藜芦醇(resveratrol,Res)混悬液组(105 mg.kg-1)、空白辅料组,以实体瘤模型小鼠的瘤重、抑瘤率观察Res-SLN对小鼠移植性肿瘤H22的抑制作用;以胸腺指数、脾脏指数为指标观察Res-SLN对免疫器官的影响;以小鼠平均生存时间观察Res-SLN对腹水瘤模型小鼠的生命延长作用。结果:Res-SLN能有效地抑制实体瘤模型荷瘤小鼠体内肿瘤的生长,低、中、高3个剂量组的抑瘤率分别为61.95%,72.39%,85.52%;Res-SLN给药组小鼠胸腺指数和脾脏指数与荷瘤对照组相比,无显著性差异;Res-SLN中、高剂量组对腹水瘤肿瘤模型小鼠的生存时间具有延长作用,与荷瘤对照组相比具显著性差异。结论:Res-SLN对荷瘤小鼠肿瘤生长具有一定的抑制作用,抑制作用强于Res,并有剂量依赖性。

Objective：To study the anti-tumor effect of resveratrol-solid lipid nanopartieles（Res-SLN）on mouse H22 hepatocellular carcinoma in vivo.Method： KM mice bearing H22 tumor cells were used in this study.It were randomly divided into tumor model group;CTX group（25 mg·kg-1）;low,middle,high dosage groups of Res-SLN（35,70,105 mg·kg-1）;suspensions group of Res（105 mg·kg-1）;auxiliary materials group.The anti-tumor activity of Res-SLN on solid tumor mice was evaluated by tumor mass weight and tumor inhibitory ratio,and the life-prolongation effect on ascitic tumor mice was assessed by average survival time.The influence on the immune organs was evaluated by the indexes of thymus and spleen of mice with transplanted tumor.Result： Res-SLN had significant inhibiting effect on transplanted tumor growth in mice.Inhibitory rates of Res-SLN in low,middle,high dose groups on H22 mice were 61.95%,72.39%,85.52%,respectively.The differences of indexes of thymus and spleen were insignificant between the ORI-SMEDDS group and model control group.Res-SLN in the middle,high doseage groups had an effect on prolonging the life of mice with transplanted tumor,the statistical difference being significant as compared with the model group.Conclusion： Res-SLN shows certain inhibition on the growth of transplanted H22 tumor with dose-dependence,and the inhibitory effect is stronger than oridonin.