槲皮素壳聚糖复合膜治疗大鼠口腔溃疡的研究

The Experimental of Quercetin Chitosan Composite Film on the Treatment of Oral Ulcer

目的:制备槲皮素壳聚糖复合药膜(quercetin chitosan composite film QUCS),观察其对口腔溃疡愈合过程的影响并探讨其作用机制。方法:以壳聚糖(chitosan,CS)为成膜材料,加入槲皮素(quercetin,QU)制成口腔溃疡复合膜。制备大鼠口腔溃疡模型,随机分为基质组(CS,不加槲皮素的膜8 mg/片)、复合膜(QUCS 0.2 mg/片)组、冰硼散(BPS 0.2 mg/溃疡面)组和空白对照组。各药均2次/d,直至溃疡完全愈合为止。观察每组溃疡鼠的一般状况、溃疡愈合速度、溃疡感染情况。观察QUCS对二甲苯所致小鼠耳肿胀及对热板法所致小鼠疼痛的影响。结果:与对照组比较,QUCS组平均愈合时间(6.24±1.35)d、平均愈合速度(3.13±0.44)mm2.d-1、溃疡周围感染程度明显缩短或减轻(P<0.01)。在溃疡表面感染程度、溃疡平均愈合时间上QUCS组和BPS组之间无统计学差异。与对照组比较,QUCS对二甲苯所致小鼠耳肿胀及对热板法所致小鼠疼痛有明显的抑制作用(P<0.01),180 min仍有强的镇痛作用。结论:QUCS对NaOH所致大鼠口腔溃疡具有明显的治疗作用,其机制可能是通过降低溃疡表面充血水肿及良好的镇痛作用实现的。

Objective：To explore the preparation of quercetin chitosan composite film,and toobserve its effect on dental ulcer repair.Method： Using chitosan as carriers,composited membrance was prepared by adding quercetin.Rabbit dental ulcer was induced by NaOH.Sixty model rats were divided into matrix group（CS,the membrance without quercetin）,composite membrance group（QUCS）,Bingpeng San group（BPS）,control group.The drugs were given 2 times a day,until the ulcer was healed.The goss,ulcer area,ulcer volume and healing speed,macroscopic were observed Edema,hyperemia and healing of ulcer surface were observed.Result：Compared with the control group,the QUCS could shorten and lessen the average healing time、average healing time、ulcer surface obviously（P0.05,P0.01）.Conclusion： QUCSFM has obvious treatment on rats oral ulcer induced by NaOH.