四叶参多糖对糖尿病大鼠血糖及免疫功能的影响

Effects of Polysaccharides Extracted from Radix Codonopsis Lanceolatae on Blood Glucose and Immune Function in Rats Diabetes with Mellitus

目的:研究四叶参多糖对糖尿病大鼠血糖及免疫功能的影响。方法:采用腹腔注射四氧嘧啶制备糖尿病大鼠模型。灌胃给予四叶参多糖(0.1,0.5 g.kg-1),于治疗0,7,14,21 d取大鼠尾血,用酶化学方法检测空腹血糖;分离胸腺、脾脏,计算胸腺指数和脾指数;MTT法检测淋巴细胞增殖能力,计算刺激指数(SI)。结果:与正常对照组大鼠比较,模型组大鼠空腹血糖显著升高,3周后,胸腺极度萎缩(P<0.01),脾淋巴细胞转化能力明显下降(P<0.01)。四叶参多糖治疗2周后,与模型组比较,大鼠空腹血糖显著降低(P<0.05),连续治疗3周,胸腺指数显著提高(P<0.05,P<0.01);高剂量组大鼠脾淋巴细胞转化能力增强,刺激指数明显升高(P<0.05)。结论:四叶参多糖可降低糖尿病大鼠空腹血糖,并能改善其细胞免疫功能。

Objective： To study the effects of polysaccharides extracted from Radix Codonopsis Lanceolatae（PCL） on blood glucose and immune function in rats with diabetes mellitus（DM）.Method： The rat DM model was established by alloxan injected intraperitoneally.The therapy group rats were administrated PCL at 0.1 or 0.5 g·kg-1 dose.The level of fasting blood glucose was detected by enzyme-chemical method on the 0,7,14,21 day of PCL therapy;the body weight and weight of thymus and spleen were measured for spleen and thymus index;lymphocyte proliferation was observed by MTT method for stimulation index（SI）.Result： Compared with normal control group,the level of fasting blood glucose of DM model group significantly increased（P0.01）,3 weeks later,the thymus extremely withered,spleen lymphocyte transformation ability obviously decreased（P0.01）.Compared with DM model group,PCL could observably reduce blood glucose after 2 weeks treatment（P0.05）.3 weeks later,thymus index of PCL therapy group enhanced significantly（P0.05,P0.01）,and SI increased obviously in the high dose PCL therapy group.Conclusion： PCL can decrease the level of fasting blood glucose in DM rats,meanwhile,cellular immune function can be improved significantly.