复方苦参注射液对小鼠的镇痛止血和抗应激作用

Effects of Compound Kushen Injection on Analgesia,Hemostasis and Anti-stress in Mice

目的:观察复方苦参注射液对小鼠的镇痛、止血作用,和对荷瘤小鼠的抗应激作用。方法:苦参注射液剂量为含生药15.0,7.5,3.8 g.kg-1,ip。以热板法、扭体法(连续给药3 d)、毛细管和断尾法(连续给药5 d)分别观察复方苦参注射液对小鼠的镇痛和止血作用,并观察连续给药10 d对肝癌荷瘤小鼠耐缺氧和低温游泳时间的影响。结果:与对照组比较,复方苦参注射液高、中剂量可明显提高小鼠的热痛阈,显著延长小鼠扭体的潜伏期,显著减少扭体次数,明显缩短小鼠的凝血和出血时间,并可明显延长肝癌荷瘤小鼠的耐缺氧时间和低温游泳时间。结论:复方苦参注射液具有明显的镇痛和止血作用,并能增强荷瘤小鼠的抗应激能力。

Objective：To observe the effects of Compound Kushen Injection on analgesia,hemostasis in mouse,and to observe to the anti-stress（induced by hypoxia and low temperature swimming） effect of the injection in tumor-bearing mice.Method： The hot plate method,the body-twisting method,capillary and broken tail method were used for evaluating the effects on analgesia and hemostasis accordingly,anti-stress effect of the injection was observed in the hepato-tumor-bearing mice.Result： Compared with controls,compound Kushen injection of 4,2,1 g·kg-1 could obviously increase the pain threshold for heat in mice,significantly extend the body-twisting incubation period and reduce the counting,obviously reduce the blood coagulation and bleeding time,and significantly prolong the hypoxia tolerance duration and low temperature swimming time in the tumor-bearing mice.Conclusion： Compound Kushen Injection has obvious analgesia and hemostatic effect,and can enhance the anti-stress ability in tumor-bearing mice.