miRNA的重要调节者Dicer和Drosha与子宫内膜异位症的相关性研究

The Main miRNA Regulators Drosha and Dicer Are Associated With Ectopic Endometrium

利用荧光定量PCR和Western blot检测证实,在异位的子宫内膜组织中Dicer和Drosha的表达低于在位子宫内膜组织,随后体外培养在位子宫内膜组织,采用siRNA干扰Dicer和Drosha,发现与干扰对照组相比,子宫内膜细胞的增殖加快而凋亡减少;同时ELISA检测显示转化生长因子-β1(transforming growth factor-beta 1,TGF-β1)的表达上调;Western blot检测显示凋亡抑制蛋白Bcl2表达增加而促凋亡蛋白Bax的表达减少.结果表明,miRNA的重要调节者Dicer和Drosha可以影响TGF-β1及Bcl2/Bax的表达进而影响细胞的增殖和凋亡,从而参与了子宫内膜异位症的形成.

Endometriosis is a common gynecological disease often resulting in chronic pelvic pain and infertility. The pathogenesis of endometriosis is likely multifactorial and several hypotheses have been suggested to explain the presence of ectopic endometrial tissue and stroma. There is a growing body of evidence indicating that miRNA is involved in the etiology of endometriosis, so this study explored the possible roles of Dicer and Drosha in endometriosis formation. Paired eutopic and ectopic endometrium in ovarian endometriosis was collected and cultured. Quantitative RT-PCR and Western blot of pairs of eutopic and ectopic endometrium demonstrated mRNA and protein levels for Dicer and Drosha in ectopic endometrium （EC） were decreased significantly compared with eutopic endometrium （ELI）. When transfected with Dicer or Drosha small hairpin RNA （shRNA） in EU, a significant increase in cell proliferation and decreased in cell apoptosis was detected. Furthermore, the Bcl2 was increased and Bax was deceased. In comparison, a scramble siRNA transfection did not affect protein levels for these molecules. Results demonstrate the low expression of Dicer and Drosha significantly correlates with EC, and shDicer or shDrosha can affect cell proliferation and apoptosis, manipulating Dicer and Drosha in EC may result in novel treatment strategies for EMs.