摘要
利用微卫星DNA分子标记对广东湛江乌石(ZJ)、广西北海山口(BH)与越南锦普(JP)3个光裸星虫(Sipunculus nudus)野生群体的遗传多样性进行分析。结果表明,20个微卫星座位中有3个位点(Snu08、Snu10、Snu14)呈现单态,17个多态微卫星座位中的等位基因数介于2~9之间,多态信息含量(PIC)介于0.097~0.797之间。每个群体中有5个位点显著偏离了Hardy-Weinberg平衡,在位点Snu18上3个群体均偏离Hardy-Weinberg平衡。3个光裸星虫野生群体均表现出中等程度的遗传多样性水平,锦普群体遗传多样性水平高于北海和湛江群体。群体间F-统计量分析表明,群体遗传分化处于低等分化水平,差异不显著(Fst=0.019~0.049,P>0.05);基于DA遗传距离构建的NJ和UPGMA聚类树均显示,地理位置相邻的群体聚在一起。研究表明,3个光裸星虫地理群体均具有中等的遗传多样性,杂合度均偏低,群体间存在较大的基因流,杂合子明显缺失;群体间已产生遗传分化,但分化水平还较低。
We evaluated the genetic diversity of three wild populations [Zhanjiang(ZJ),Beihai(BH),Jingpu(JP)] of Sipunculus nudus in the Beibu Gulf using 20 microsatellite DNA loci.We calculated the allele numbers,effec-tive allele numbers,expected heterozygosity,observed heterozygosity and Hardy-Weinberg equilibrium.Three loci were monomorphic and 17 loci were polymorphic.Five loci in each population deviated significantly from Hardy-Weinberg equilibrium.The locus Snu18 exhibited significant deviation from Hardy-Weinberg equilibrium in all 3 populations.Though diversity was higher in JP than in BH and ZJ,Fst analysis across the stocks and loci revealed a low level of divergence.We constructed NJ and UPGMA clustering trees based on DA genetic distance.The populations from nearby geographical locations were clustered together in both trees.
出处
《中国水产科学》
CAS
CSCD
北大核心
2012年第1期62-69,共8页
Journal of Fishery Sciences of China
基金
国家科技支撑计划项目(2007BAD29B03)
广东省自然科学基金资助项目(06029125)
广东海洋大学面上项目(0812069)
关键词
北部湾
光裸星虫
微卫星
遗传多样性
Beibu Gulf
Sipunculus nudus
microsatellite
genetic diversity