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磁性活性细胞分选技术在男性不育研究中的应用 被引量：3

Magnetic activated cell sorting and its application in the studies of male infertility
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摘要 磁性活性细胞分选法(MACS)根据细胞表面特异的标记物,在分子水平对目的细胞进行有效分选,具有简易、快速、灵活、特异性高的特点,在临床方面有着广泛的应用。MACS也为男性不育提供了一个新的研究平台,将MACS用于精液质量优化与生殖细胞分离是男性不育研究的一个新思路。本文简要介绍了MACS的基本原理,综述了MACS在精子优选、冷冻保存、精原干细胞及生精细胞分离等男性不育研究方面的应用现状和临床应用前景。 Magnetic activated cell sorting（MACS） is considered as a flexible,fast,specific and simple cell sorting system that can separate target cells effectively according to specific markers on the cell surface,for which it has won a wide clinical application.MACS offers a new platform for male infertility research,as well as a novel idea for applying this technology in the optimization of semen quality and the isolation of germ cells.This article briefly introduces the basic principles of MACS,and summaries its present and potential clinical application in male infertility research,as in spermatozoa selection and cryopreservation,and the isolation of spermatogonial stem cells and germ cells.
作者 邓雪连 林发全
机构地区 广西医科大学第一附属医院检验科
出处 《中华男科学杂志》 CAS CSCD 2012年第1期70-73,共4页 National Journal of Andrology
基金 广西科学研究与技术开发计划项目(桂科攻10124001A-45)~~
关键词 磁性活性细胞分选法 男性不育 精子优选 生殖细胞分离 magnetic activated cell sorting male infertility spermatozoa selection isolation of germ cells
分类号 R698.2 [医药卫生—泌尿科学]
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参考文献28

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二级参考文献23

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共引文献12

同被引文献50

  • 1周庆辉,周新华,张泉,王得志,张良,黎莉.整合素α_6亚基在不同年龄段小鼠睾丸精原细胞表达观察[J].中国组织化学与细胞化学杂志,2005,14(2):147-150. 被引量：1
  • 2李延仓,李孝建,沈雁.表皮干细胞标记物鉴定及应用现状[J].中国组织工程研究与临床康复,2007,11(46):9329-9332. 被引量：4
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引证文献3

二级引证文献16

中华男科学杂志
2012年 第1期

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