苦参/白头翁不同配比乙醇提取物体外杀精的实验研究

Spermicidal effect of alcohol extracts from different ratios of Sophora flauescens Ait/Chinese Bulbul in vitro

目的:研究不同配比的苦参/白头翁乙醇提取物的体外杀精能力。方法:收集15例正常男性的精液标本,经密度梯度离心法处理,与9种不同配比的苦参/白头翁乙醇提取物一起孵育20 s、2 min及4 min后,采用计算机辅助的精液分析系统(CASA)检测精子活力及运动参数;同时分别检测其具有瞬间杀精能力时的最低有效浓度。结果:以3∶1配比的乙醇提取物,浓度为0.5 mg/ml时能明显抑制精子活力,并且对精子运动参数VCL、VSL、VAP、ALH、WOB及MAD等指标均产生了明显的抑制作用,与对照组比较有统计学意义;9个不同配比的乙醇提取物中以3∶1配比时,其具有瞬间杀精能力时的最低有效浓度最低,为3.5 mg/ml。结论:苦参/白头翁3∶1配比时,其乙醇提取物具有最佳的体外杀精能力。

Objective： To observe the spermicidal effect of alcohol extracts from different ratios of Sophora flauescens Ait / Chinese Bulbul in vitro.Methods： Semen samples aseptically obtained by masturbation and prepared by density gradient centrifugation from 15 healthy men were incubated in the alcohol extracts from 9 different ratios of Sophora flauescens Ait / Chinese Bulbul for 20 seconds,2 minutes and 4 minutes.Then the motility and movement parameters of the sperm were detected by computer-assisted semen analysis,and the minimal effective concentrations of the instant spermicidal effect of the extracts were determined.Results： At the ratio of 3∶ 1,the extract at 0.5 mg/ml significantly inhibited the sperm motility and other sperm movement parameters VCL,VSL,VAP,ALH,WOB and MAD,as compared with the control group.The minimal effective concentration of the instant spermicidal effect of the extracts was 3.5 mg/ml at 3∶ 1.Conclusion： The alcohol extracts from Sophora flauescens Ait and Chinese Bulbul at the ratio of 3∶ 1 have the best spermicidal effect in vitro.