葡萄糖浓度影响寡发酵链球菌抑制变形链球菌作用的研究

Influence of glucose concentration on the inhibition of Streptococcus oligofermentans on Streptococcus mutans

目的研究不同葡萄糖浓度对寡发酵链球菌（Streptococcus oligofermentans，So）与变形链球菌（Streptococcus mutans，Sm）之间相互作用的影响，及对so产过氧化氢能力的影响。方法通过平板培养法观察在不同葡萄糖浓度下So与Sm之间的相互作用；运用4．氨基安替吡啉-辣根过氧化物酶法测定不同葡萄糖浓度下So过氧化氢的初始产生速率和产量。结果环境葡萄糖浓度为0、10、50mmoL／L时均可见so对Sm有抑制作用；Sm受抑制区面积占菌膜面积比值：同时接种so和Sm时，无糖环境比值为0．202±0．005，10、50mmol／L葡萄糖环境分别为0．467±0．025和0．468±0．028；先接种So再接种Sm时，无糖环境比值为0．394±0．004，10mmol／L葡萄糖环境为0．811±0．075，50mmol／L葡萄糖环境为0．816±0．007。葡萄糖浓度为10、50mmoL／L时So对Sm的抑制作用均较无糖环境下显著（P〈0．05），但两种浓度抑制作用差异无统计学意义（P〉0．05）。葡萄糖浓度为10、50mmol／L时So的过氧化氢初始产生速率[（23．573±0．263）、（23．337±0．473）umol·L-1·min-1]均显著高于无糖环境[（10．513±0．516）umol．L-1·min-1]，P〈0．05。无糖环境下So对数生长期各时段过氧化氢产量高于有糖环境（P〈0．05）。1000mmol／L葡萄糖环境下未见So抑制Sm，亦未能检测到So产生过氧化氢。结论So抑制Sm的能力受糖环境的影响，在10、50mmol／L葡萄糖环境下，So具有更强的抑制Sm能力。

Objective To investigate the inhibition of Streptococcus oligofermentans （So） on Streptococcus mutans （Sm） and the producibility of hydrogen peroxide by So under the influence of glucose concentration environment. Methods The inhibition between So and Sm was observed by plating method under the different glucose concentration environment. The initial synthesis rates and production of hydrogen peroxide by So were determined under the different glucose concentration environment by 4-aminoantipyinehorseradish peroxidase method at A510. Results Under 0, 10 and 50 mmol/L glucose environment, the inhibition of So on Sm was evident. When both Sm and So were inoculated at the same time, the ratio of inhibition area by bacterial membrane area was 0. 202 ± 0. 005,0. 467 ± 0. 025,0. 468 ± 0. 028 under 0,10, 50 mmol/L glucose environment. When So was cultivated first and then Sm applied, the ratio was 0. 394 ± 0. 004, 0. 811 ± 0. 075 and 0. 816 ± 0. 007 under 0,10 and 50 mmol/L glucose environment respectively. The inhibition under 10 and 50 mmol/L glucose environment were more significant than that under nonglucose environment. There was no significant difference between these two glucose concentrations （P〉0.05）. The initial synthesis rates of H2O2 by So under the 10 mmol/L [（23.573 ± 0. 263） umol · L-1 · min-1] and 50 mmol/L [ （23. 337 ± 0. 473 ） umol· L-1 · min-1 ] glucose were higher than without glucose[ （ 10. 513 ±0. 516） umol· L-1 ·min-1 ] ,P 〈0. 05. H2O2 was not detected in 1000 mmol/L glucose. However, the production of H2O2 by So under 0 mmol/L glucose was higher than other glucose concentrations （ P 〈 0. 05 ）. Conclusions The capability of the inhibition of So on Sm was affected by glucose environment and was much stronger under certain glucose concentrations（ 10,50 mmol/L）.