CD147-shRNA对SMMC-7721细胞增殖和运动能力的影响

Inhibitory effect of CD147-shRNA on proliferative and migration ability of SMMC-7721 cells

目的:探讨CD147-shRNA重组质粒对肝癌细胞SMMC-7721增殖和运动能力的影响。方法:将前期合成并筛选的重组质粒pBS/U6/CD147-shRNA3转染至SMMC-7721细胞中,采用MTT法检测细胞增殖;采用划痕愈合实验测定细胞迁移能力。结果:MTT实验结果显示0.3μg和1μg的pBS/U6/CD147-shRNA3转染后,SMMC-7721细胞的增殖受到明显抑制,且呈浓度依赖性,最大抑制率为32.2%;转染对照载体的SMMC-7721细胞在划痕24 h后趋于愈合,相比之下,转染pBS/U6/CD147-shRNA3的SMMC-7721细胞划痕愈合能力明显减弱。结论:基因沉默CD147基因对肝癌细胞SMMC-7721的增殖和运动有抑制作用。

Objective： To investigate the effect of the recombinant plasmid containing CD147-shRNA on the proliferative and migration ability of SMMC-7721 cells. Methods： The previously constructed and screened recombinant plasmid pBS/U6/CD 147-shRNA3 was transfected into the SMMC-7721 cells. After that, the proliferation and migration ability of SMMC-7721 cells were detected by MTT and wound healing assay, respectively. Results： Xhe MqT results showed that the proliferation of SMMC-7721 cells was significantly inhibited in a concentration dependent manner after transfection with pBS/U6/CD147-shRNA3 （0.3 and 1 μg）, and the maximum percent inhibition was 32.2%. The scratch wound closure of the SMMC-7721 cells transfected with the control plasmid was almost complete after 24 hours and, in contrast, the scratch wound healing capability of the SMMC-7721 cells transfected with pBS/U6/CD147-shRNA3 was much weakened. Conclusion： CD 147 gene silencing has inhibitory effect on the proliferation and migration of SMMC-7721 ceils.