S期激酶相关蛋白2在肝再生组织中的表达及其意义

Role of S-phase kinase-associated protein 2 in liver regeneration after partial hepatectomy

目的:观察S期激酶相关蛋白2(Skp2)在大鼠肝部分切除后残肝中的表达,探讨其在肝再生过程中的意义。方法:大鼠行70%肝部分切除术,分别在术后12,24,48,72,168 h,用免疫组化方法检测肝再生组织中Skp2及PCNA蛋白表达。结果:肝部分切除术后12,24,48,72 h,大鼠残肝组织中的Skp2及PCNA均表达明显增高,与相应各时间点的假手术组比较差异均有统计学意义(均P<0.01),且两者均在肝切除后48 h表达量达到最高峰,呈明显正相关(r=0.9085,P<0.05)。结论:Skp2在肝部分切除术后肝再生中起重要作用。

Objective： To observe the expression of S-phase kinase-associated protein 2 （Skp2） in the remnant liver tissue of rat with partial hepatectomy so as to determine its role in liver regeneration. Methods： Rats underwent 70% partial heptectomy and the their remnant liver tissues were collected at 12, 24, 48, 72 and 168 h after respectively pectively, after surgery. The expressions of Skp2 and proliferating cell nuclear antigen （PCNA） protein in the regenerating liver tissue were detected by immunohistochemical staining. Results： The expressions of Skp2 and PCNA in the remnant liver tissue of the rats with partial hepatectomy were all significantly higher than those of the sham operated rats at the observation time of 12, 24, 48 and 72 h, respectively after surgery （all P〈0.01）. Both the Skp2 and PCNA expression reached their maximal levels at 48 h after hepatectomy and there was a significant correlation between them （r=0.9085, P〈0.05）. Conclusion： Skp2 plays an important role in liver regeneration after partial hepatectomy.