摘要
目的:探讨晚期糖基化终产物受体(RAGE)基因多态性对中国汉族人群冠状动脉(冠脉)病变狭窄程度的影响。方法:应用多聚酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测109例冠脉轻中度狭窄(mild/moderate coronary stenosis,MCS)患者、134例重度狭窄(serious coronary stenosis,SCS)患者和170例健康对照者的RAGE基因3种多态性(-429T/C、1704G/T和G82S)基因型。结果:RAGE基因G82S多态性的S等位基因在SCS组的分布显著高于对照组(27.6%vs 13.2%,OR值为2.086,95%CI为1.494~2.914,P<0.001,Pcorr<0.003),而MCS组与对照组、MCS组与SCS组间差异无统计学意义(P>0.05,Pcorr=0.069);多变量Logistic回归分析提示与单倍体型T-Gly-T相比,单倍体型G-Ser-T发生冠脉SCS的危险显著增加(OR值为1.961,95%CI为1.150~3.343,P=0.013)。其余2种多态性(-429T/C和1704G/T)的基因型及等位基因频率分布在各组间差异无统计学意义(P>0.05)。结论:携带有RAGE基因G82S多态性的单倍体型发生冠脉SCS的危险性显著增加,提示该单倍体型与冠脉SCS的发生呈明显相关性。
Objective:To investigate whether polymorphisms of the RAGE gene were associated with the degree of coronary artery stenosis Chinese Han patients.Method:The allele frequencies and genotype distribution combinations of the-429T/C,1704G/T and G82S polymorphisms of the RAGE gene were compared in 109 cases of mild/moderate coronary stenosis(MCS),134 cases of serious coronary stenosis(SCS) and 170 control subjects.PCR-RFLP was used for detection of genotypic variants.Result:The S allele frequency of the G82S polymorphism was higher in the SCS(odds ratio ,2.086;95% CI,1.494~2.914;P0.001,Pcorr0.003) group when compared with the control group.However,the S allele frequency was not significantly different between the MCS and the SCS patient groups(P0.05,Pcorr=0.069),and there was no significant difference of allele frequency distributions was observed in the MCS group(P=0.075).Haplotype-based logistic regression analysis revealed that haplotype G-Ser-T(OR,1.961;95% CI,1.150~3.343;P=0.013),compared to the reference haplotype T-Gly-T,was associated with an increased risk of SCS after adjusting for other risk factors.Conclusion:The haplotype carrying the G82S variant of the RAGE gene was significantly associated with an increased risk of SCS.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2012年第1期41-45,共5页
Journal of Clinical Cardiology
基金
广东省"211工程"项目"器官衰竭的防治研究"基金资助