吡喹酮透皮给药治疗日本血吸虫病的效果观察

Transdermal delivery of praziquantel for treatment of Schistosoma japonicum adult worms

目的本文旨在探究吡喹酮(Praziquantel,PZQ)透皮剂不同透皮剂量和次数的治疗效果,以及吡喹酮透皮剂对发育成熟前后日本血吸虫的治疗效果,为日本血吸虫病提供更加便利的治疗途径。方法试验中每组昆明小鼠均感染日本血吸虫尾蚴40±2条,感染后21d、28d和35d采用不同透皮剂量的吡喹酮透皮剂和不同透皮次数治疗昆明小鼠,各试验组均设对照组,感染后42d解剖冲虫,计算减虫率、减肝卵率、减毛蚴孵化率和减雌率,评价透皮剂的治疗效果。结果感染日本血吸虫35d后吡喹酮透皮剂透皮1次高剂量减虫率为56.92%,其它剂量透皮1次减虫率都在50%以下,低剂量透皮3次减虫率为56.15%;减肝卵率都在70%以下;对21d、28d透皮3次的减虫率都在65%以上,减肝卵率基本接近100%。结论 PZQ透皮制剂对日本血吸虫有一定的杀灭作用,杀灭效果具有剂量和次数依赖性,少量多次治疗效果较好。

This article aims to explore the fiiectiveness of transdermal agent of PZQ by different dose and treat times,and ist praziquantel transdermal agent on Schistosoma japonicum around mature,providing more convenient access to treatment of schistosomiasis.All of the experiment groups of Kunming mice were infected with 40±2 Schistosoma japonicum cercariae 21d,28d and 35d.They were treated through abdominal and transdermal patches with different dose and treat times;While all of the experiment groups set control groups,the mices were all dissected 42d after infection.The worm reduction rate,egg reduction rate,miracidia hatching reduction rate and female reduction rate of treatment groups were calculated and the therapeutic effect of transdermal agent was evaluated.Results indicated that 35 d after infection,the worm reduction rate for single high-dose of praziquantel transdermal patch was 56.92%;the worm reduction rates for single dose of other dosages were all less than 50%;the worm reduction rate for three-time lose-dose was 56.15%;the egg reduction rates in liver were all less than 70%.21 d and 28 d after infection,the worm reduction rate for three-time transdermal patch were all more than 65%;the egg reduction rates in liver were all close to 100%.It＇s concluded that praziquantel transdermal patch has certain effectiveness on killing of Schistosoma japonica,whose effect is depend on dosage and frequency.Repeated small dose of drug use would lead to a better treatment effect.