薄皮甜瓜高效再生体系的建立

Establishment of High Frequency Shoot Regeneration System of Cucumis melo

对"G24"和"甘甜一号"薄皮甜瓜材料5～7d苗龄的子叶、下胚轴、根尖、真叶以及不同6-BA浓度下不定芽诱导情况进行了研究,建立了高效稳定的再生体系并对生根以及移栽等条件进行了初步研究。结果表明:2份薄皮甜瓜材料在不定芽的诱导方面差异不大,"G24"不定芽的发生频率达到97.2%,"甘甜一号"为91.6%;最适宜作为甜瓜组织培养的外植体材料为子叶近胚轴端,其不定芽诱导率可达95%以上;不定芽诱导中最适6-BA浓度为1.0mg/L。

Cotyledon, hypocotyledonary axis, root tip, euphylla and adventitious buds inducement with various concentration of 6-BA of ＇G24＇ and ＇Gantian No. 1＇of Cucumis rnelos at the growing stage of 5-7 d were studied in this research,in order to establish stable tissue culture techniques for genetic transformation and to provide technical support. At the meantime,conditions for root growth and transplant were also primarily studied. The results showed that no significant difference on adventitious buds inducement with the two tested material. The adventitious buds inducement rate of ＇G24＇ was 97. 2~,while ＇Gantian No. 1＇ was 91.6%;cotyledon at the hypocotyl side was the optimum explants for tissue culture with over 95 % of adventitious buds inducement rate and the optimum concentration of 6-BA was 1.0 mg/L.