摘要
以水为溶剂,以提取时间、温度和料液比3个因素作为研究对象,以多糖提取量为评价指标,在单因素的基础上,通过三因素三水平的Box-Behnken响应面分析法研究了优化多糖的提取工艺条件。结果表明:最优提取条件为提取时间1.43h、温度76.8℃、料液比1∶80.3,在此条件下,多糖得率可达到4.11mg/g。提取液经一系列纯化工艺后,得到纯度为61.32%多糖。
Polysaccharides in Neosinocalamus a f finis （Rendle） Keng f. levels was extracted by water to produce. Singlefactor method was adopted to investigate the effects of extract-time, temperature and solid-liquid ratio on degree of extraction content of polysaccharides. Subsequently, a central composite design（CCD） involving 17 experiments of three variables（i, e., extract-time, temperature and solid-liquid ratio） at three levels combined with response surface methodology was employed to attain the highest extraction content. The results showed that the optimal extract-time, temperature, solid-liquid ratio were 1.43 h, 76. 8℃, 1 ： 80. 3, respectively. On this condition, the content of polysaccharides was 4. 11 mg/g. By the method of a series of purification process,the purity of polysaccharides was 61.32%.
出处
《北方园艺》
CAS
北大核心
2012年第2期167-171,共5页
Northern Horticulture
基金
西南林业大学校级科研专项资助项目(110959)
关键词
慈竹
多糖
响应面
脱色
纯化
Neosinocalamus affinis （Rendle） Keng f.
polysaccharides
response surface methodology
decoloration
purification
