宁夏惠农区回、汉族人群肿瘤坏死因子α-308位点基因多态性与胃癌前疾病及胃癌相关性研究

Association of TNF-α-308 gene polymorphism with gastric precancerrous diseases and gastric cancer

目的研究宁夏惠农区回、汉族人群肿瘤坏死因子(Tumor Necrosis Factor,TNF)-α-308位点基因多态性与慢性萎缩性胃炎、胃溃疡及胃癌的相关性,明确宁夏惠农区回、汉族人群TNF基因多态性与胃癌易感性的关系。方法采用聚合酶连反应-限制性片段长度多态性(PCR-RFLP)方法检测宁夏惠农区回、汉族383例正常对照者、慢性萎缩性胃炎、胃溃疡和胃癌患者中TNF-α-308位点基因多态性,其中正常对照组99例(回族45例,汉族54例)、慢性萎缩性胃炎组97例(回族45例,汉族52例)、胃溃疡组75例(回族35例,汉族40例)和非贲门胃癌组112例(回族50例,汉族62例)。结果回、汉族TNF-α-308位点A/A、G/G、A/G基因型及A、G基因频率在正常对照组、慢性萎缩性胃炎、胃溃疡和胃癌中差异无统计学意义(P>0.05)。结论惠农区回、汉族TNF-α-308位点基因多态性与胃癌前疾病及胃癌发生无明显关系。

Objective To study on association of Tumor Necrosis Factor-alpha（TNF-α）-308 gene polymorphism in chronic gastritis,gastric ulcer and gastric cancer in Hui and Han nationalities of Ningxia area,and to discuss effect between genetic factor of parasitifer with chronic gastritis,gastric ulcer and gastric cancer,and to identify the relationship between TNF gene polymorphism and hereditary susceptibility in gastric cancer.Methods TNF-α-308 gene polymorphism were detected by the PCR-RFLP method in 383 cases with gastric precancerous diseases,gastric cancer and healthy controls.Among 97 cases chronic gastritis（45 Hui nationalities,52 Han nationalities）,75 cases with gastric ulcer（35 Hui nationalities,40 Han nationalities）,112 cases gastric cancer（50 Hui nationalities,62 Han nationalities） and 99 healthy controls（45 Hui nationalities,54 Han nationalities）.Results Frequency of TNF-α-308 A/A,G/G,A/G genes and A,G genotypes in healthy controls,gastric precancerous diseases and gastric cancer had no significant difference by statistical comparison in Hui and Han nationalities（P〉0.05）.Conclusion TNF-α-308 gene polymorphism could not be associated with gastric precancerous diseases and gastric cancer in Hui and Han nationalities in Ningxia.