摘要
目的:建立氟氯西林镁阿莫西林干混悬剂有关物质检查的HPLC方法.方法:采用Dikma Diamonsil-C18(4.6×250 mm,5μm),对氟氯西林镁和阿莫西林分别进行测定.氟氯西林镁的有关物质检查以磷酸盐缓冲液(pH5.0)-乙腈(67:33)为流动相;检测波长225nm;阿莫西林的有关物质检查以磷酸盐缓冲液(pH5.0)-乙腈(98:2)为流动相;检测波长254nm;柱温40℃.结果:各降解产物均可与主药进行良好分离,方法的专属性和精密度良好.结论:该方法准确、简便、快速,结果可靠.
Objective： To establish an HPLC method for determination of related substances in Flucloxacillin Magnesium and Amoxicillin Oral Suspension.Method： A Dikma Diamonsil C18 column（250mm×4.6mm,10μm） is used.The mobile phase is phosphoric buffer（pH 5.0）-acetonitrile（67：33） and the wavelength of UV detection is 225nm for the determination of related substances of Flucloxacillin Magnesium.The mobile phase is phosphoric buffer（pH 5.0）-acetonitrile（98：2）,the wavelength of UV detection is 254nm and the column temperature is 40℃ for the determination of related substances of Amoxicillin.Result： The peaks of degradations could be well separated from the main peak.The specificity and precision are good.Conclusion： This method is simple,quick,accurate and effective to determinate related substances in Flucloxacillin Magnesium and Amoxicillin Oral Suspension.
出处
《西南民族大学学报（自然科学版）》
CAS
2012年第1期92-96,共5页
Journal of Southwest Minzu University(Natural Science Edition)
基金
国家外专局项目(2011-33)
