滇藁本中黄酮类化合物的提取工艺

Extraction Process for Flavonoids in Yunnan Ligusticum

目的:研究彝药滇藁本总黄酮的最佳提取工艺。方法:以槲皮素为对照品,HPLC测定的槲皮素含量和总固体物收率为指标,考察提取方法加醇量、提取时间、乙醇浓度对滇藁本总黄酮提取率的影响。结果:加醇量、提取时间、乙醇浓度对滇藁本药材粗粉的槲皮素含量和总固体物收率都有显著的影响,而提取时间影响最显著。最佳提取工艺为药材与70%乙醇按照1:8(g/mL)的比例,75℃回流提取3h。结论:本实验优选出了滇藁本中黄酮的最佳提取工艺,为滇藁本中黄酮类化合物的进一步研究提供依据。

Objective: To study the best extraction process for flavonoids in Yunnan Ligusticum.Methods: Taking quercetin as reference substance and the quercetin content determined by HPLC and its total solids yield as indicators,observations were made to study the extraction method,the amount of increasing alcohol,the extraction time and the impact of ethanol concentration on the total extraction rate of the flavonoids in Yunnan Ligusticum.Results: The amount of increasing alcohol,the extraction time and the ethanol concentration have a significant impact on the quercetin content in Ligusticum medicine meal and the total solids yield whereas the extraction time have the most significant impact.The best extraction process was that the ratio of herbs and 70% of ethanol was by 1:8(g/mL),with a back extraction 3h at 75 ℃.Conclusion: The best extraction process for the flavonoids in Yunnan Ligusticum from the experiment provides a basis for further research.