HPLC法测定金不换各部位中大黄酸、大黄素含量

Determination of Rhein and Emodin in Rumex obtusifolius L.by HPLC

采用高效液相色谱法测定河南栽培金不换中大黄酸、大黄素的含量,色谱柱为Hy-persilC18柱(250mm×4.6mm×5μm),流动相:甲醇∶0.25%磷酸(体积比80∶20),流速:1.1mL/min,检测波长为436nm.结果:大黄酸在0.154～1.54μg/mL、大黄素在0.412～2.06μg/mL范围内其峰面积积分值与质量浓度之间呈良好的线性关系(r=0.999 6和0.999 9),平均回收率分别为100.2%、99.87%(n=6),RSD分别为1.47%、2.1%.该方法简便、准确,可用于金不换中大黄酸、大黄素含量的测定.

The research establishes a HPLC method for the determination of Rhein and Emodin in Rumex obtusifoli- us L grown in Henan. Hypersil C18 （250mm×4.6mm×5μm）and mobile phase methanol -phosphoric acid （80：20 ） were used. The flow rate was 1.1 mL/min and the UV detection was 436 nm. The result showed that the linear range of Rhein concentration was from 0. 154 μg/mL to 1.54 μg/mL （ r = 0. 9996 ）, while Emodin was between 0. 412 -2. 06μg/mL , and its linear relation was good （ r =0. 9996 and 0. 9999）. The average recovery rate was 100.2% , and 99.87% （n =6） with the RSD of 1.47% and 2. 1%. The method is simple, accurate, reproduci- ble and applicable to the determination of the content of Rhein and Emodin in Rumex obtusifolius L..