6-位脂肪胺取代的萘酰亚胺衍生物与DNA的相互作用

Interaction between 6-Aliphatic Amine Substituted Naphthalimide Derivatives and DNA

利用紫外可见光谱、荧光光谱、圆二色谱和粘度测试,考察了8个6-位脂肪胺取代的萘酰亚胺衍生物与小牛胸腺DNA的相互作用.研究结果表明:小牛胸腺DNA对与其结合能力强的化合物3a～3d有荧光猝灭作用,对与其结合能力弱的3e～3h有荧光增强效应;圆二色谱显示这些萘酰亚胺化合物能引起DNA二级结构发生不同的变化.在紫外-可见吸收光谱和粘度测试中,这些化合物与DNA的相互作用没有差别.综合光谱学和粘度测试的结果,推断这些化合物是通过嵌插方式与DNA结合,由于结构不同,它们与DNA之间作用程度存在差异.

The DNA-binding behavior of eight 6-aliphatic amine substituted naphthalimide derivatives was investigated by UV-vis absorption spectra,fluorescence spectra,circular dichroism（CD） and viscosity measurement.The significant fluorescence quenching of 3a,3b,3c and 3d having stronger DNA-binding efficiency was observed upon addition of DNA,while the significant fluorescence enhancement of 3e,3f,3g and 3h having weaker DNA-binding efficiency was seen.CD results indicated that the change the secondary DNA structure induced by the derivatives was different.In UV-vis and viscosity measument,no obvious difference in the interaction of the derivatives and DNA was found.Taking the spectra and viscosity measurement results,it was concluded that the naphthalimide derivatives bound to DNA via intercalation and there was differential in the degree of the interaction.