模拟移动床色谱法纯化白藜芦醇

Purification of Resveratrol by Simulated Moving Bed Chromatography

采用三带模拟移动床色谱法纯化虎杖白藜芦醇。实验结果表明:模拟移动床色谱纯化白藜芦醇的工艺参数为:流动相为V(甲醇):V(水):1:1;4根IDL=10mm×200mm ODS制备柱;柱连接方式1-2-1;洗脱带流量1.0mL/min,精制带流量1.5mL/min,吸附带流量1.6mL/min,进样流量0.1mL/min,切换时间19min。

Purification of resveratrol from polygonum cuspidatum was accomplished with three zones simulated moving bed chromatography.The parameters of purification for resveratrol with simulated moving bed chromatographic were as follows：mobile phase of methanol-water（1：1,V/V）, 4 columns（IDL= 10mm×200mm,ODS stationary phase）,columns connection of 1-2-1,the refine zone flow of 1.5mL/min,the extraction zone flow of 1.0mL/min,the sorption zone flow of 1.6mL/min,the injection sample flow of 0.lmL/min and the switch time of 19min.