微波消解ICP-MS测定尿样中硒及其他重金属元素

Determination of Selenium and Other Heavy Metals in Human Urine by ICP-MS with Microwave Digestion

建立微波消解电感耦合等离子体-质谱法(ICP-MS)同时测定尿样中硒及铬、镉、砷、铅、锰、钴、镍、铜、锌10种重金属元素的方法。采用硝酸-过氧化氢消解体系,微波消解法制备样品.以铟、铋为内标物质,直接用ICP-MS测定上述10种元素。所测10种元素的检出限为0.006-0.073μg/L,校准曲线线性关系好(r>0.9999),相对标准偏差(RSD)均小于3%,对尿样标准物质的测定值均在标准参考值范围内。应用微波消解ICP-MS分析尿样,方便快捷,灵敏度高,检出限低,重现性好,是理想的生物样本检删分析方法。

The method for the determination of selenium and other heavy metals,such as chromium,manganese,cobalt,nickel,cadmium,lead,arsenic,copper and zinc,in human urine was established by inductively coupled plasma-mass spectrometry（ICP-MS） with microwave digestion. The samples were prepared by microwave digestion with HNo_3-H_2O_2 digestion system,and the 10 kind of elements were directly determined by ICP-MS with indium and bismuth as internal standard substance.The detection limits of the 10 elements were in the range of 0.006—0.073μg/L,and the standard curve had the good linear relationship with correlation coefficient above 0.9999 and RSD less than 3%,and the measured value of urine standard substance were all in the standard reference ranges.The urine samples were analyzed by ICP-MS with microwave digestion,and the method is simple and fast with high sensitivity,low detection limit and good repeatability,that is an ideal method for analysis and detection of biological samples.