摘要
建立微波消解电感耦合等离子体-质谱法(ICP-MS)同时测定尿样中硒及铬、镉、砷、铅、锰、钴、镍、铜、锌10种重金属元素的方法。采用硝酸-过氧化氢消解体系,微波消解法制备样品.以铟、铋为内标物质,直接用ICP-MS测定上述10种元素。所测10种元素的检出限为0.006-0.073μg/L,校准曲线线性关系好(r>0.9999),相对标准偏差(RSD)均小于3%,对尿样标准物质的测定值均在标准参考值范围内。应用微波消解ICP-MS分析尿样,方便快捷,灵敏度高,检出限低,重现性好,是理想的生物样本检删分析方法。
The method for the determination of selenium and other heavy metals,such as chromium,manganese,cobalt,nickel,cadmium,lead,arsenic,copper and zinc,in human urine was established by inductively coupled plasma-mass spectrometry(ICP-MS) with microwave digestion. The samples were prepared by microwave digestion with HNo_3-H_2O_2 digestion system,and the 10 kind of elements were directly determined by ICP-MS with indium and bismuth as internal standard substance.The detection limits of the 10 elements were in the range of 0.006—0.073μg/L,and the standard curve had the good linear relationship with correlation coefficient above 0.9999 and RSD less than 3%,and the measured value of urine standard substance were all in the standard reference ranges.The urine samples were analyzed by ICP-MS with microwave digestion,and the method is simple and fast with high sensitivity,low detection limit and good repeatability,that is an ideal method for analysis and detection of biological samples.
出处
《光谱实验室》
CAS
CSCD
2012年第1期320-323,共4页
Chinese Journal of Spectroscopy Laboratory
基金
2010年科研院所技术开发研究专项资金(2010EG166314)