摘要
目的研究CCN1基因在人肾癌组织中的表达变化,初步探讨其与肾癌发生的关系。方法对42例开放手术切除的肾癌组织标本进行免疫组织化学染色,应用IPP图像分析软件对染色结果进行定量分析;运用RT-PCR技术检测肾癌组织标本中CCN1mRNA的表达,应用Quantity one图像分析软件对RT-PCR结果进行定量分析。18例距离癌区2cm以上的正常肾组织标本作为对照组,均经病理检查证实为正常组织。结果免疫组织化学结果显示CCN1基因在肾癌组织及正常肾组织中均有表达,但其在肾癌组织中的表达强度显著高于正常肾组织(P<0.01)。RT-PCR结果显示肾癌组织中CCN1mRNA的表达显著多于正常肾组织(P<0.01)。结论 CCN1基因在肾癌组织中表达增高,可能与肾癌的发生有重要关系。
Objective To study the expression change of CCN1 in kidney cancer. Methods Kidney cancer tissue specimens were produced from 42 patients undergoing surgical ablation, and 18 normal kidney tissue specimens got from the area far away form the tumor. Immunohistochemical staining was performed to detect the expression of CCN1 in kidney tissue,and RT-PCR technique was used to detect the expression of CCN1 mRNA. Results The expression of CCN1 mRNA and protein in kidney cancer group were much higher than that in normal kidney tissue group （P〈0.01）. Conclusion The expression of CCN1 gene is significantly in- creased in kidney cancer tissues, which may have an important role during the development of kidney cancer.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第3期244-245,248,F0003,共4页
Chongqing medicine
基金
重庆市卫生局基金资助项目(06-2-067)
