绝经后妇女肾虚证与MTHFR基因多态性相关性研究

Correlation of Kidney Deficiency Syndrome with Methylenetetrahydrofolate Reductase Gene Polymorphism in Postmenopausal Women

目的:探讨绝经后妇女肾虚证与亚甲基四氢叶酸还原酶(methylenetetrahydrofolate reductase,MTHFR)基因多态性的内在联系。方法:以100例绝经后肾虚证妇女为研究对象(分为单纯肾虚组和疾病肾虚组各50例),另设健康妇女50例为对照组,运用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测MTHFR基因型和等位基因频率,对结果进行分类统计学分析。结果:单纯肾虚组、疾病肾虚组MTHFR C677T的CT基因型及T等位基因频率显著高于对照组,差异均有非常显著性意义(P<0.01)。疾病肾虚组的CT基因型及T等位基因频率高于单纯肾虚组,差异均有非常显著性意义(P<0.01)。结论:MTHFR基因突变可能参与肾虚发展成老年性疾病的致病过程。

Objective. To explore the correlation of kidney deficiency syndrome in postmenopausal women with methylenetetrahydrofolate reductase （MTHFR） gene polymorphism . Methods： One hundred postmenopausal women with kidney deficiency syndrome were equally randomized into kidney deficiency（KD）group and kidney deficiency complicated with senile diseases（KDD）group. Fifty healthy volunteers served as the control group. MTHFR genotype and allele frequencies were detected by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）. The results were analyzed by classification statistic. Results： The frequency of MTHFR C667 CT genotype and T allele in KD group and KDD group was higher than that in the control group, the differences being significant（P 〈 0.01）. The frequency of MTHFR C667 CT genotype and T allele in KDD group was higher than that in KD group, the differences being significant（P 〈 0.01）, Conclusion. MTHFR gene mutation may be involved in the development of senile diseases from kidney deficiency.