化浊解毒方含药血清对胃癌细胞VEGF及COX-2表达的影响

Effect of Serum Containing Huazhuo Jiedu Formula on VEGF and COX-2 Expression in Human Gastric Cancer Cell Lines

目的:研究化浊解毒方对胃癌细胞血管内皮生长因子(VEGF)及环氧合酶-2(COX-2)表达的调控作用。方法:以不同剂量的化浊解毒方给Wistar大鼠灌胃制备含药血清,然后以含药血清培养胃癌细胞,共同培养2代后,分别用免疫组化法检测VEGF、COX-2的表达情况。结果:化浊解毒方高、中、低剂量组胃癌细胞中VEGF、COX-2的表达减弱,与对照组比较,差异均有显著性意义(P<0.01);而且高剂量组表达减弱更为明显,与低、中剂量组与较,差异均有非常显著性意义(P<0.01)。结论:化浊解毒方对胃癌细胞VEGF及COX-2的表达有抑制作用。

Objective, To investigate the regulatory effect of Huazhuo Jiedu Formula on vascular endothelial growth factor （VEGF） and cyclooxygenase-2（COX-2） expression in human gastric cancer cells. Methods, Serum containing Huazhuo Jiedu Formula was prepared by gastric infusion with different dosages of Huazhuo Jiedu Formula to Wistar rats. And then the drug-containing serum was used to cultivate gastric cancer cells for two generations. VEGF and COX-2 expression levels were detected by immunohistochemical method. Results, VEGF and COX-2 expression levels in Huazhuo Jiedu Formula groups was decreased（P 〈 0.01 compared with those in the control group）, and the decrease was most obvious in high-dose Huazhuo Jieclu Formula group （P 〈 0.01 compared with those in low- and moderate-dosage Huazhuo Jiedu Formula group）. Conclusion. Huazhuo Jiedu Formula has inhibitory effect on the expression of COX-2 and VEGF in human gastric cancer cells.