新生大鼠原代心肌细胞的培养

Cultivation of original cardiac myocyte from neonate rats

目的高效获得乳鼠原代心肌细胞。方法取新生24 h乳鼠心脏,剪碎后单独用质量分数为0.08%的胰蛋白酶进行多次消化、细胞洗涤获得心肌细胞。台盼蓝染色后观察心肌细胞存活率。以搏动细胞数占视野中细胞总数比例估测心肌细胞纯度。结果获得的原代心肌细胞存活率>85%、纯度>95%。结论通过上述方法可获得较高存活率及纯度的原代心肌细胞,该培养方法简单易行,重复性好,基本能满足实验要求。

Objective To obtain cardiac myocyte efficiently. Methods The heart of 24 hours neonate rat was obtained and made it smashed,then it was digested by 0.08% trypsinase for many times and was washed by culture media to collect cardiac myocyte. The cell survival rate was estimated after trypan blue tintaged and the purity of cardiac myocyte was estimated by the proportion of beating cells account for the total ceils in visual field. Results By the methods, the cell survival rate was more than 85% and the purity was more than 95%. Conclusion The high cell survival rate and purity can achieve by this way. The process is simple and have fine repetitiveness and can satisfy the request of experiment.