热毒宁注射液对哮喘大鼠肺组织病理改变及TRAF2表达的调控作用

Effect of Reduning Parenteral Solution on the Expression of Tumor Necrosis Factor Receptor Associated Factor 2 and Pathological Modification in Asthmatic Rat Lung Tissue

目的:观察热毒宁注射液对哮喘大鼠肺组织肿瘤坏死因子受体相关因子(TRAF2)表达和病理改变的影响,并探讨其可能的作用机制。方法:SD大鼠27只随机分成哮喘组、对照组和热毒宁组,每组9只。哮喘组和热毒宁组建立大鼠哮喘模型,光镜下观察肺组织病理改变,免疫组织化学法检测肺组织TRAF2的表达。结果:HE染色显示哮喘组肺内支气管及血管壁周围见大量炎性细胞浸润、黏液腺增生,气道上皮断裂和脱落、黏液栓形成。热毒宁组炎性细胞数目较哮喘组明显减少,黏膜上皮增生不明显,支气管腔见较少的炎性分泌物,黏液腺增生不明显,未见黏液栓形成。免疫组化结果显示,哮喘组支气管壁TRAF2的光密度值(0.317±0.041)显著高于对照组(0.220±0.057)(P<0.01);热毒宁组支气管壁TRAF2的光密度值(0.291±0.019)与哮喘组相比差异无统计学意义(P>0.05),仍显著高于对照组(P<0.01)。结论:哮喘大鼠TRAF2的表达水平增强,其可能参与了哮喘的气道炎症过程。热毒宁能减轻哮喘大鼠气道炎症,可能不是通过TRAF2途径。

Objective：To investigate the potential roles of Reduning,a Chinese Herb parenteral solution, in the ex pressions of tumor necrosis factor receptor associated factor 2（TRAF2） and pathological changes in lung tissues in asthmatic rats. Methods： A total of 27 SD rats were randomly divided into asthma group,control group,and Redun- ing-treated group,9 in each.Asthmatic model was established in the asthma and Reduning-treated groups. Pathologi- cal changes of lung tissue was observed under light microscope. The expressions of TRAF2 protein was detected by immunohistochemical method. Results： HE staining showed that in the asthma group, inflammatory cells were infil- trated in lung tissue, surrounding of bronchial wall, and blood vessel wall,and mucous gland was hyperplasia, air- way epithelium was broken and desquamated, and mucus plug was shaped. In the Reduning-treated group, all the above mentioned pathological changes were attenuated. Immunohistochemisty showed that both the expression of TRAF2 protein in lung tissue in the asthma group （0.317±0.041 ）and in the Reduning-treated group （0.291 ＋0.019 ） were significantly higher than that in the control group（0.220＋0.057,all P〈0.01）. No significant difference in TRAF2 protein expressions between the asthma group and the Reduningtreated group was noted （P〉O.05）. Conclusion： Levels of TRAF2 were increased in asthmatic rats, and it may paly a potential role in pathogenesis of asthma inflammation. Reduning can down-regulate airway inflammation of asthmatic rats, the underlying mechanism may not involve TRAF2 signal transduction pathway.