益肾导痰调冲汤对多囊卵巢综合征大鼠瘦素及其受体和IGF-IR表达的影响

Effect of Yishendaotantiaochong Decoction on Leptin and Leptin Receptor and Insulin-like Growth Factor I Receptor in Polycystic Ovary Syndrome Rats

目的:观察益肾导痰调冲汤对多囊卵巢综合征(PCOS)大鼠瘦素及其受体和胰岛素样生长因子I受体(IGF-IR)表达的影响。方法:SD大鼠,采用"T+HCG"造模法建立PCOS大鼠模型。随机分为正常对照组、空白模型组、益肾导痰调冲汤等效剂量组(以下简称中药组)、西药达英-35组、达英加中药组、桂枝茯苓丸组各10只。观察卵巢组织形态学改变;放射免疫法测定血清内分泌水平;ELISA法测定瘦素(Lep);免疫组化法测定卵巢组织瘦素受体(OB-R)及IGF-IR表达。结果:益肾导痰调冲汤可改善PCOS大鼠卵巢组织结构,缩小卵巢体积,减少囊状卵泡及闭锁卵泡数目。降低LH、LH/FSH、T、Lep,增高E2水平,降低OB-R、IGF-IR阳性表达(P<0.05或P<0.01)。结论:益肾导痰调冲汤可改善PCOS大鼠lep与OB-R的结合障碍,调节OB-R、IGF-IR的生物利用度,从而改善PCOS大鼠的高雄激素血症及排卵障碍。

Objective： To observe the effect of Yishendaotantiaochong decoction（YSDTTCD）on the expression of rat leptin and its receptor （OB-R） and insulin-like growth factor I reeeptor（IGF-IR） with polycystic ovary syndrome （PCOS）. Methods： Rat models with PCOS were established by hypodermic injection with T and HCG. SD rats were randomly divid- ed into 6 groups： normal control,model matched control,YSDTTCD-treated group t,Diane-35 pill-treated group, YSDTTCD and Diane-35-treated group, and Guizhifuling pill-treated group. The ovaries were harvested from the rats for histomorphological observation. The levels of serum endocrine index were determined by radio-immunity. Serum leptin was deter- mined by ELISA. OB-R and IGF-IR in the tissues of ovaries was assessed with immunohistochemistry method. Results： By treatment with YSDTrCD the ovarian volume and the number of vesicular follicle and atretic follicle in ovarian tissue were decreased, the levels of LH, LH/FSH,T, and leptin were downregulated, and the serum level of E2 was increased. The expression of OB-R and IGF-IR were reduced in the tissue of ovaries（P〈0.05 or P〈0.01）. Conclusion：YSDTFCD may interrupt the combination of leptin and OB-R in PCOS rats and regulate the biological availability of OB-R and IGF-IR in ovaries, to improve high serum-T and ovulation barrier.