外阴阴道念珠菌感染及其耐药性

Candida infection of vulvovagina and its drug-resistance

目的了解女性外阴阴道真菌种类及其对药物的敏感性。方法回顾性分析1107例标本,对其中的258例阳性标本的菌株用科玛嘉念珠菌显色培养基鉴定,用Rosco纸片法对5-氟胞嘧啶、氟康唑、两性霉素B、制霉菌素、咪康唑、克霉唑进行体外药物敏感性试验。结果 1107例临床标本共检出念株菌258株,阳性率为23.3%。其中,白念珠菌占82.6%,光滑念珠菌占6.6%,热带念珠菌占3.5%,克柔念珠菌占2.3%,其他念珠菌占5.0%。念珠菌对制霉菌素、两性霉素B和5-氟胞嘧啶的敏感性较高,分别为97.3%、93.0%和88.0%;对咪康唑和氟康唑的耐药率较高,分别为50.4%和43.8%。结论不同菌种对药物的敏感性不同;对外阴阴道感染的念珠菌进行菌种鉴定和药敏试验对临床治疗有重要意义。

Objective To investigate the vulvovaginal infection with candida and its drug-resistance. Methods A total of 1107 samples was retrospectively analyzed,of which 258 candida positive strains were identified by CHROM agar candida and the tests of sensibility to 5-fluorocytosine, flueonazol,amphotericin B,nystatin,miconazole and clotrimazole were performed by Rosco disk difusion method. Results Of 1107 clinical samples,258 samples（23.3%） were positive for candidas.In the positive samples,candida albicans accounted for 82.6%,candida glabrata 6.6%,candida tropicalis 3.5%,candida krusei 2.3% and the other candidas 5.0%,respectively.The results of the tests of sensibility to drugs showed that candida was more sensitive to nystatin,amphotericin B and 5-fluorocytosine with the sensitive rates of 97.3%,93.0% and 88.0%,respectively,and had higher drug-resistance to miconazole and fluconazol with the drug-resistant rates of 50.4% and 43.8%, respectively. Conclusion Various candidas have diferent sensibility to drugs. Identification of candidas and sensibility tests of candidas to drugs should be performed before the treatment.