静息态功能磁共振低频振幅技术评价睡眠剥夺下针刺三阴交后效应

Evaluation of the post-effect of acupuncture at Sanyinjiao(SP 6) under sleep deprivation by resting-state amplitude of low-frequency fluctuation:a fMRI study

目的:探讨三阴交作用机制及与非穴低频振幅(ALFF)变化脑区的差异。方法:16名健康志愿者分别进行2次在睡眠剥夺(SD)24h后接受针刺,第1次取右侧三阴交穴(简称SP 6组),第2次取非穴(即平三阴交后方1寸处,简称非穴组),针刺后静留针4min。2次针刺间隔2周,同时3.0TMR采集数据,REST软件计算脑区ALFF值,用SPM 5对组内单样本及组间配对t检验进行分析,分别得到相应ALFF变化图。结果:SP 6组主要引起顶下小叶、后扣带回皮质ALFF值变化;非穴组主要引起楔前叶、后扣带回皮质ALFF值变化;SP 6组相对非穴组仅引起颞梭回、额中回ALFF值增高,无减低区。结论:两组SD状态静留针对静息态脑网络活动均有影响,情绪相关脑区表现明显,其作用机制可能是通过相应的作用中枢及多个脑区共同构成的复杂脑功能网络对靶器官进行调节从而发挥相应功效。针刺经穴与非穴存在差异性。

Objective To discuss the central modulating mechanism of Sanyinjiao（SP 6） and the amplitude of low-frequency fluctuation（ALFF） differences of the functional brain regions between SP 6 and sham acupoint.Methods Sixteen volunteers accepted right Sanyinjiao（SP 6）（SP 6 group） or right sham（sham group） acupuncture for two times after 24 h sleep deprivation（SD）,there was two weeks between two acupunctures.The needle was retained for 4 minutes and the brain fMRI scanning was performed by 3.0TMR every time.The ALFF consequent data was processed by REST.One-sample t-test and two-pair t-test were performed by SPM5.Results SP 6 group mainly caused ALFF change in inferior parietal lobule,posterior cingulated while sham group in precuneus,posterior cingulated.SP 6 group increased ALFF in left fusiform temporal gyrus,medial frontal gyrus with no decreased regions compared with sham group.Conclusions Retaining needle dose influence the activity of the brain region during resting-state in both group,especially the mood-related regions.And its mechanism is probably that the relevant effect is carried out by regulating function to target organs of complex brain network comprised of relevant functional center and related brain region.There are differences between SP 6 and Sham acupoint.