艾燃烧生成物对快速老化模型小鼠SAMP8血清抗氧化酶的影响

Effect of Artemisia burning products on antioxidant enzymes in serum of SAMP8 mouse

目的:探讨艾燃烧生成物(即艾烟)的抗衰老作用及其适宜干预参数。方法:采用析因设计,将70只快速老化小鼠(SAMP8)随机分为模型组(M组)和6个艾烟干预组:低浓度15min组(A1组)、低浓度30min组(A2组)、中浓度15min组(B1组)、中浓度30min组(B2)、高浓度15min组(C1组)、高浓度30min组(C2组),每组10只;10只正常老化小鼠(SAMR1)为正常组(Z组)。6个艾烟干预组分别用相应浓度和时间的艾烟进行熏灸。观察小鼠血清丙二醛(MDA)含量,超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性的变化。结果:模型组MDA的含量高于正常组(P<0.05),SOD和GSH-Px的活性则低于正常组(均P<0.05);艾烟干预各组各项指标或与模型组相当,或优于模型组。MDA含量和GSH-Px活性以B1组为优,SOD活性以B2组为优。时间因素无意义,浓度因素有意义,两因素对SOD和GSH-Px的影响存在交互作用。结论:一定浓度和时间的艾烟可通过提高机体抗氧化能力、减少自由基代谢产物而起到显著的抗衰老作用。艾烟干预以中浓度30min为宜。

Objective To explore the anti-aging effect of artemisia burning products（ie.smoke of moxibustion） and its proper intervention parameters.Methods According to factorial experiment design,70 SAMP8 mice were randomly divided into one model group（group M） and 6 intervention groups：low concentration with 15 min group（group A1）,low concentration with 30 min group（group A2）,middle concentration with 15 min group（group B1）,middle concentration with 30 min group（group B2）,high concentration with 15 min group（group C1）,high concentration with 30 min group（group C2）.There were 10 cases in each group.Ten age-matched SAMR1 mice were used as normal group（group Z）.All the mice in the 6 intervention groups were fumed with artemisia burning products of different concentration and time.The content of serum malondialdehyde（MDA）,the activity of superoxide dismutase（SOD） and glutathione reductase（GSH-Px） were tested.Results MDA content in group M was significantly higher than that in group Z（both P0.05）,while SOD and GSH-Px activity were significantly lower in group M than that in group Z（both P0.05）.Results of MDA,SOD and GSH-Px in 6 intervention groups were either of no statistically significant differences,or better than that in group M.Among 6 intervention groups,results of MDA and GSH-Px were better in group B1,while the result of SOD was better in group B2.Time factor didn＇t make any difference,while concentration of artemisia burning products is meaningful.As to SOD and GSH-Px,there＇s a strong interaction between the two factors.Conclusion With certain concentration and time period,the intervention of artemisia burning products can exert anti-aging effect by increasing antioxydative capability and reducing metabolites of free radicals.Middle concentration and 30 minutes are recommended when intervened with artemisia burning products.