期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

Brn-4基因修饰骨髓间质干细胞移植对老年痴呆鼠学习记忆的影响 被引量：2

下载PDF
导出
摘要 目的探讨Brn-4基因修饰大鼠骨髓间质干细胞(MSC)移植后对老年痴呆鼠学习记忆的影响。方法实验分为3组:正常组、模型组和移植组。以单侧切断穹隆海马伞(FF)建立老年性痴呆模型鼠;应用脂质体将表达载体转染到MSC中,荧光显微镜观察该Brn-4基因在MSC中的表达;将成功转染Brn-4基因修饰的MSC植入模型鼠尾静脉,4 w后行Y迷宫测试,并行免疫组化结合图像分析技术观察各组大鼠基底前脑p75阳性神经元数,同时采用电生理技术检测离体海马CA1区长时程增长效应(LTP)的诱导变化。结果 Brn-4基因在MSC中获得成功表达;免疫组化表明模型组大鼠p75阳性神经元数在内侧隔阂和斜角带较正常组明显下降(P<0.01);移植组p75阳性神经元数较模型组有改善(P<0.05)。离体海马CA1区移植组LTP在移植后6 d,12 d,30 d均较模型组有显著增强(P<0.05)。Y迷宫测试结果显示,大鼠的学习记忆能力与基底前脑p75阳性神经元数呈正相关。结论 Brn-4基因修饰MSC移植后提高了老年痴呆鼠学习记忆能力。
作者 陈艳 宣爱国 方继荣
机构地区 广州医学院荔湾医院 广州医学院人体解剖学教研室
出处 《中国老年学杂志》 CAS CSCD 北大核心 2012年第1期82-85,共4页 Chinese Journal of Gerontology
基金 广州市医药卫生科技项目(No.2009-YB-174)
关键词 Brn-4基因 骨髓间质干细胞 老年痴呆 学习记忆
分类号 Q291 [生物学—细胞生物学]
  • 相关文献

参考文献10

  • 1Gleichgerrcht E, Ibanez A, Roea M,et al. Decision-making cognition in neurodegenerative diseases[J]. Nat Rev Neurol,2010 ;6( 11 ) :611-23.
  • 2Shimazaki T,Arsenijevic Y,Ryan AK,et al. A role for the POU-Ⅲ transcription factor Brn-4 in the regulation of striatal neuron precursor differentiation[J]. EMBO J,1999;18(2) :444-56.
  • 3Sia KC, Chong WK, Ho IA, et al. Hybrid herpes simplex virus/Epstein- Barr virus amplicon viral vectors confer enhanced transgene expression in primary human tumors and human bone marrow-derived mesenchymal stem cells[J].J Gene Med .2010 : 12 (10):848-58.
  • 4陈克明,葛宝丰,刘兴炎,白孟海,王勇.纤维蛋白用做骨组织工程载体并修复骨缺损[J].中国矫形外科杂志,2005,13(16):1241-1243. 被引量：11
  • 5Kawabata K, Sakurai F, Koizumi N, et al. Adenovirus vector-mediated gene transfer into stem cells [ J ]. Mol Pharm ,2006;3 (2) :95-103.
  • 6Emura M, Ochiai A, Horino M, et al. Development of myofibroblasts from human bone marrow mesenchymal stem cells cocultured with human colon carcinoma ceils and TGF betal [J]. In Vitro Cell Dev Biol Anim,2000; 36(2) :77-80.
  • 7Sanchez-Ramos J, Song S, Cardozo-Pelaez F, et al. Adult bone marrow stromal cells differentiate into neural cells in vitro[J]. Exp Neurol,2000; 164(2):247-56.
  • 8Deng W, Obrocka M, Fischer I, et al. In vitro differentiation of human marrow stromal cells into progenitors of neural cells by conditions that increase intracellular cyclic AMP [J]. Biochem Biophys Res Commun, 2001 ;282( 1 ) :148-52.
  • 9项鹏,夏文杰,张丽蓉,陈振光,张秀明,李艳,李树浓.胞内cAMP浓度的增加可诱导骨髓间质干细胞分化为神经元样细胞[J].中国病理生理杂志,2002,18(11):1324-1327. 被引量：8
  • 10贾延劼,杨于嘉,宋元宗.黄芩甙体外诱导大鼠骨髓基质细胞成为神经细胞[J].中国病理生理杂志,2002,18(3):247-249. 被引量：23

二级参考文献17

  • 1Bensaid W, Triffitt JT, Blanchat C, et al. A biodegradable fibrin scaffold for mesenchymal stem cell transplantation [ J ]. Biomaterials,2003,24: 2497 - 2502.
  • 2Jaiswal N, Hayneworth SE, Caplan AI. Osteogenic differentiation of purified culture-expanded human mesenchymal stem cells in vitro [J]. J Cell Biochem,1998,295 -312.
  • 3Stem S,Lindenhayn K, Schultz O, et al. Cultivation of porcine cells from the nucleus pulposus in a fibrin/hyaluronic acid matrix[ J]. Acta Orthop Scand ,2000,71 (5): 496 -502.
  • 4Bicano P, Riminucci M, Gronthos S. Bone marrow stromal stem cells:nature, biology, and potential applications [ J ]. Stem Cells, 2001,19(3): 180-192.
  • 5Colter DC,Class R,DiGirolamo CM,et al.Rapid expansion of recycling stem cells in cultures of plastic- adherent cells from human bone marrow[J].Proc Natl Acad Sci USA,2000,97(7): 3213-3218.
  • 6Paolo B,Pamela GR.Marrow stromal cells [J].J Clin Invest,2000,105 (12): 1663-1667.
  • 7Sanchez RJ,Song S,Cardozo PF,et al.Adult bone marrow stromal cells differentiate into neural cells in vitro [J].Exp Neurol,2000,164(2): 247-256.
  • 8Kopen GC,Prockop DJ,Phinney DG.Marrow stromal cells migrate throughout forebrain and cerebellum,and they differentiate into astrocytes after injection into neonatal mouse brains [J].Proc Natl Acad Sci USA,1999,96(19): 10711-10716.
  • 9Shieh DE,Liu LT,Lin CC.Antioxidant and free radical scavenging effects of baicalein,baicalin and wogonin[J].Anticancer Res,2000,20(5A): 2861-2865.
  • 10Woodbury D,Schwarz EJ,Prockop DJ,et al.Adult rat and human bone marrow stromal cells differentiate into neurons [J].J Neurosci Res,2000,61(4): 364-370.

共引文献37

同被引文献45

  • 1陆明,刘丽梅.翼螺旋转录因子Foxa2与糖脂代谢及糖尿病关系的研究进展[J].上海交通大学学报（医学版）,2011,31(4):497-500. 被引量：3
  • 2梁晨,郭世文.全反式维甲酸对NT2细胞系胶质瘤趋向性的影响[J].西安交通大学学报（医学版）,2012,33(4):470-473. 被引量：3
  • 3李殿启,杨甲梅,殷正丰.FGF-20:成纤维细胞生长因子家族新成员[J].生命的化学,2006,26(5):445-448. 被引量：8
  • 4赵焕英,包金风,赵春礼,杨慧,徐群渊.应用载体介导的RNA干扰技术抑制Nurr1基因在PT67细胞中的表达[J].神经解剖学杂志,2007,23(2):121-126. 被引量：2
  • 5Tan X,Zhang L,Qin J,et al.Transplantation of neural stem cells co-transfected with Nurr1 and Brn4 or treatment of Parkinsonian rats[J] .Int J Dev Neurosci,2013,31(1):82-87.
  • 6Sousa KM,Mira H,Hall AC,et al.Microarray analyses support a role for Nurr1 in resistance to oxidative stress and neuronal differentiation in neural stem cells[J] .Stem Cells,2007,25 (2):511-519.
  • 7Kim TE,Lee HS,Lee YB.Sonic hedgehog and FGF8 collaborate to induce dopaminergic phenotypes in the Nurr1-overexpressing neural stem cell[J] .Biochem Biophys Res Commun,2003,305 (4):1040-1048.
  • 8Grothe C,Timmer M,Scholz T,et al.Fibroblast growth factor-20 promotes the differentiation of Nurr1-overexpressing neural stem cells into tyrosine hydroxylase-positive neurons[J] .Neurobiol Dis,2004,17(2):163-170.
  • 9Shimada H,Yoshimura N,Tsuji A,et al.Differentiation of dopaminergic neurons from human embryonic stem cells:modulation of differentiation by FGF-20[J] .J Biosci Bioeng,2009,107(4):447-454.
  • 10Lei Z,Jiang Y,Li T,et al.Signaling of glial cell line-derived neurotrophic factor and its receptor GFRα1 induce Nurr1 and Pitx3 to promote survival of grafted midbrain-derived neural stem cells in a rat model of Parkinson disease[J] .J Neuropathol Exp Neurol,2011,70(9):736-747.

引证文献2

二级引证文献2

中国老年学杂志
2012年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部