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自体骨髓间充质干细胞移植对心肌梗死后兔Ryanodine受体及其稳定蛋白表达的影响 被引量：4

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摘要 目的研究骨髓间充质干细胞(MSCs)移植对心肌梗死后兔ryanodine受体(RyR2)及其稳定蛋白(FKBP12.6)表达的影响,探讨MSCs移植治疗缺血性心力衰竭的机制。方法采用结扎冠状动脉前降支的方法制备新西兰白兔缺血性心力衰竭动物模型。利用密度梯度离心法和贴壁法获得MSCs,经5-氮胞苷诱导后,4',6-二脒基-2-苯基吲哚(DAPI)标记。移植后4 w,实时荧光定量RT-PCR法检测梗死边缘区RyR2和FKBP12.6 mRNA表达。采用超声观察术前及术后左心室射血分数(LVEF)的变化。结果与对照组比较,AMI组梗死边缘区心肌RyR2和FK-BP12.6 mRNA下降(P<0.05)。与AMI组比较,MSCs组梗死边缘区心肌RyR2和FKBP12.6 mRNA增加(P<0.05)。结论 MSCs移植使RyR2和FKBP12.6表达增加是治疗缺血性心力衰竭的机制之一。
作者 胡炜 李树仁 张素巧 乔建晶 王天红 刘东霞 荀丽颖 齐晓勇
机构地区 河北省人民医院 河北医科大学附属河北省人民医院
出处 《中国老年学杂志》 CAS CSCD 北大核心 2012年第1期110-113,共4页 Chinese Journal of Gerontology
关键词 急性心肌梗死 心力衰竭 间充质干细胞 RYANODINE受体 FKBP12.6
分类号 R542.2 [医药卫生—心血管疾病]
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参考文献13

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同被引文献44

  • 1王曼,李树仁.基因工程在骨髓间充质干细胞治疗心肌梗死中的应用[J].中国老年学杂志,2015,35(1):269-271. 被引量：4
  • 2周青,郭瑞强,陈新军,陈金玲.经胸超声心动图对3种兔心力衰竭模型的检测[J].中国超声医学杂志,2005,21(5):325-327. 被引量：7
  • 3Winter J,Brack KE,Ng GA. cardiac contractility modulation in the treatment of heart failure:initial results and unanswered questions[J].European Journal of Heart Failure,2011.700-710.
  • 4Zhang J,Qi XY,Wan YF. Establishment of a reperfusion model in rabbits with acute myocardial infarction[J].Cell Biochemistry and Biophysics,2011.249-258.
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  • 6Borgreffe M,Burkhoff D. Clinical effects of cardiac contractility modulation(CCM)as a treatment for chronic heart failure[J].European Journal of Heart Failure,2012.700-710.
  • 7Brunckhorst CB,Shemer I,Mika Y. Cardiac contractility modulation by non-excitatory currents:studies in isolated cardiac muscle[J].European Journal of Heart Failure,2006,(1):7-15.doi:10.1016/j.ejheart.2005.05.011.
  • 8Imai M,Rastogi S,Gupta RC. Therapy with cardiac contractility modulation electrical signals improves left ventricular function and remodeling in dogs with chronic heart failure[J].Journal of the American College of Cardiology,2007,(21):2120-2128.doi:10.1016/j.jacc.2006.10.082.
  • 9Gupta RC,Mishra S,Wang M. Cardiac contractility modulation electrical signals normalize activity,expression,and phosphorylation of the Na+-Ca2+ exchanger in heart failure[J].Journal of Cardiac Failure,2009.48-56.
  • 10van den Borne SW, Diez J, Blankesteijn WM, et al. Myo- cardial remodeling after infarction: the role of myofibro- blasts[J]. Nat Rev Cardiol, 2010, 7(1):30-37.

引证文献4

二级引证文献8

中国老年学杂志
2012年 第1期

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