慢性乙型肝炎肝细胞凋亡与肝脏微循环障碍的关系研究

The relationship between hepatocyte apoptosis and liver microcirculation disturbance

目的研究慢性乙型肝炎肝细胞凋亡与肝脏微循环障碍的关系。方法采用TUNEL法检测肝细胞的凋亡率,同时采用透射电镜观察肝细胞超微结构的变化和肝窦微循环结构的改变。结果慢性乙型肝炎肝细胞凋亡率显著增加,细胞膜皱缩并分裂成多个凋亡小体,肝窦内皮细胞窗孔减小减少并伴有狄氏腔胶原沉积和基底膜形成,同时可发现WP小体。结论慢性乙型肝炎肝细胞凋亡是肝脏微循环障碍的重要促进因素。

Objective To study the relationship between hepatocyte apoptosis and liver microcirculation disturbance. Method Hepatocyte apoptosis was detected by TUNEL method. Ultrastructure of hepatocyte and the changes of sinus hepaticus microcirculation construction were observed by transmission electron microscope （TEM）. Result The rate of hepatocyte apoptosis of patients with chronic hepatitis B increased obviously ; hepatocyte cytolemma shrinked and divided to many apoptosis bodies. Decreased sizes and reduced numbers of sinusoidal endothelial cells＇ （SECs） fenestrae and collagen deposited in Disse space; basal lamina can be found on SECs and WP bodies were found in SECs. Conclusion Hepatocyte apoptosis is an important promoting factor to liver microcirculation disturbance in patients with chronic hepatitis B.