胰岛素抵抗大鼠脂肪组织chemerin mRNA的表达和意义

Expressions and Significance of chemerin mRNA in Adipose Tissue of Insulin Resistant Rats

目的探讨胰岛素抵抗大鼠脂肪组织中脂肪因子chemerin mRNA的表达情况及其与胰岛素抵抗之间的关系。方法采用高脂饲料喂养建立胰岛素抵抗大鼠模型,将30只雄性SD大鼠随机分为正常饮食组(NC,n=15)和高脂饮食组(HF,n=15),喂养10周后,以大鼠清醒状态下正常血糖-高血浆胰岛素钳夹技术进行评估,处死后取肾周脂肪组织,应用Real-time PCR方法检测大鼠脂肪组织中chemerin mRNA的表达水平。结果 (1)与NC组比较,HF组60～120 min的平均葡萄糖输注率(GIR60～120)明显降低[分别为(13.87±1.45)mg·kg-·1min-1和(24.10±2.87)mg·kg-·1min-1,P<0.05];(2)与NC组比较,HF组大鼠脂肪组织中chemerin mRNA的表达明显升高[分别为(1.86±1.02)和(0.92±0.32),P<0.01]。结论胰岛素抵抗大鼠脂肪组织chemerin mRNA升高,提示脂肪因子chemerin可能参与胰岛素抵抗的发生。

Objective To investigate the expressions of chemerin mRNA in adipose tissue of insulin resistant rats and the relationship be- tween them. Methods Insulin resistant rats were induced by high-fat diet feeding. Thirty male SD rats were randomly divided into two groups fed with either basic chow （NC, n = 15 ） or high-fat diet （HF, n =15 ） for 10 weeks, then assessed by euglycemic-hyperinsulinemia clamp technique. Rats were sacrificed and taken the perinephrit adipose tissue.The chemerin mRNA expression in adipose tissue was tested by real-time PCR. Results （1） The glucose infusion rate （GIR60-120） was significantly decreased in HF group compared with control group,GIR60-120 （13.87＋-1.45 mg.kg-1.min-1 vs 24.10＋-2.87 mg.kg-1.min-1 ,P 〈 0.05）. （2）The chemerin mRNA expression in adipose tissue was significantly increased in HF group compared with control group （ 1.86＋-1.02 vs 0.92＋-0.32,P 〈 0.01 ）. Conclusion The increased expression of chemerin mRNA in adipose tissue of insulin resistant rats showed that cheraerin might be involved in insulin resistance.