从甜叶菊叶片中分离纯化抗氧化内生真菌

Separation and Purification of Antioxidant Endophytic Fungi from the Stevia Leaves

[目的]从甜叶菊叶片中分离纯化抗氧化内生真菌。[方法]以甜叶菊叶片为材料,从中分离纯化出内生真菌,对它们进行了液体培养,检测其发酵上清液的总抗氧化性能、清除DPPH自由基和抑制化学发光的能力。[结果]共分离获得42株内生真菌,编号为F01-F42。总抗氧化最强的为F41;清除DPPH能力最强的为F37;综合比较总抗氧化性能和清除DPPH能力,F02、F07、F20、F25、F37、F41 6株较突出。利用流动注射化学发光法对这6株发酵液进行检测,化学发光抑制率均大于70%,验证了它们的抗氧化性能较突出;对这6株进行了初步形态鉴定,它们的菌落颜色为白色或淡黄色,F02菌丝有隔膜,其他没有。[结论]筛选出高抗氧化活性的真菌,为将来的抗氧化研究奠定了基础。

[Objective] The aim was to separate and purify antioxidant endophytic fungi from Stevia leaves.[Method] Stevia leaves were used as materials for endophytic fungi screening and purification.Then,liquid culture was conducted to test fermented supernatant abilities of antioxidant,DPPH free radical clearing and chemiluminescence inhibition.[Result] Forty two endophytic fungi were separated from F01 to F42.F41 was proved to be the strongest in antioxidant aspect;F37 was the strongest in DPPH clearing;F02,F 07,F20,F25,F37,and F416 stood out in a comprehensive comparison of the above aspects.Flow injection chemiluminescenct method was used to test the above six fermentation liquors,and the results showed that antioxidant abilities of the six were quite strong for all of chemiluminescence rates were over 70%.Through preliminary morphological identification,bacterial colony was found white or yellowy and only F02 was of dissepiment.[Conclusion] Fungi with high-antioxidation activities were separated,which paved the way for future research.