摘要
[目的]综述microRNA(miRNA)表达检测技术的研究进展,为miRNA表达研究及阐明其调控功能奠定基础。[方法]通过概述miRNA表达水平检测技术的原理、特点及进展,说明如何简便有效地检测其表达。[结果]miRNA是一类重要的非编码小RNA分子,调控一系列重要生命过程,其表达研究是研究其调控功能的重要途径,用于检测miRNA表达的方法主要有Northern blot、原位杂交(In Situ Hybridization,ISH)、表达谱芯片、RT-PCR以及克隆测序。[结论]该研究为简便有效地检测miRNA表达提供了方法,为阐明其对动植物重要生命过程的调控机理奠定了基础。
[Objective] The aim was to summarize the research progress of microRNA(miRNA)expression detection technique,to lay the foundation for miRNA expression study and it's functional control.[Methods] Through summarizing the principles,characteristics and progress of miRNA expression level detection technique,to explain how to detect it's expression simply and effectively.[Result] miRNA is an important class of small non-protein coding RNA,regulate and control a series of important life processes,it's expression study is the important approach for the study of it's control function,the detection method for miRNA expression are Northern blot,In Situ Hybridization,ISH,Expression Profile Chip,RT-PCR and cloning and sequencing.[Conclusion] The study provides methods for detecting miRNA expression simply and effectively,and lay the foundation for expounding it's control mechanism for important life processes of animals and plants.
出处
《安徽农业科学》
CAS
2012年第3期1346-1347,1351,共3页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金(30901025)
湖北省重点实验室项目(2011ZD123)