猪α干扰素表达蛋白ab的抗病毒效果研究

Study on the Antiviral Effect of the Expression Protein ab of Porcine Interferon-α

[目的]探索猪α干扰素表达蛋白ab的抗病毒效果。[方法]通过测定猪α干扰素(PoIFN-α)表达的融合蛋白ab对接种水泡性口炎病毒(VSV)的牛肾细胞(MDBK)保护作用的效价,来研究其抗病毒效果。通过中和试验,测定保护50%细胞不产生病变所需PoIFN-α表达的融合蛋白ab的稀释度。[结果]PoIFN-α表达的融合蛋白ab具有显著的抗病毒作用,稀释度1∶4.9的2.0μg/ml ab蛋白溶液即可保护50%细胞不产生细胞病变。[结论]猪重组α干扰素具有高活性、纯度高、无毒副作用、无药物残留等特点,其研制与开发对猪生产实践和疾病防治具有重要意义。

[Objective] The research aimed to explore the antiviral effect of the expression protein ab of porcine interferon –α（PoIFN-α）.[Method] The titer of the protective effect of the fusion protein of PoIFN-α to MDBK cells inoculated with vesicular stomatitis virus（VSV）was determined to study its antiviral effect.Using neutralization test,the dilution of fusion protein ab of PoIFN-α that protected 50% of MDBK cells from lesion was determined.[Result] The fusion protein ab expressed by PoIFN-α had significant antiviral effects and 2.0 μg/ml protein ab solution with the dilution of 1∶4.9 could protect 50% of MDBK cells from cellular diseases.[Conclusion] PoIFN-α had high activity,high purity,no toxic and side effect,no drug residue and other characteristics.And its development and exploitation was of significance for the production practice and disease control of pigs.