恒河猴血管内皮细胞移植替代角膜内皮细胞后房水血管内皮生长因子水平的变化

Change of vascular endothelial growth factor in aqueous humor after rhesus monkey corneal endothelial cells substituted by vascular endothelial cells

目的探索恒河猴血管内皮细胞移植替代角膜内皮细胞后房水血管内皮生长因子(vascular endothelial growth factor,VEGF)浓度的变化。方法实验组将体外培养增殖的恒河猴视网膜-脉络膜血管内皮细胞通过离心沉淀法移植到撕除后弹力层的恒河猴角膜内表面;对照组撕除角膜内皮层后,不做任何处理,直接原位缝合角膜植片。分别于术前及术后1周、2周、3周、4周、6周、8周、12周抽取房水,通过ELISA方法检测其中VEGF浓度,并进行统计分析。结果实验组和对照组术后1周时VEGF浓度差异无统计学意义(P>0.05),术后2周、3周、4周时实验组VEGF浓度分别为(374.74±3.30)ng·L-1、(419.06±1.39)ng·L-1、(481.83±3.36)ng·L-1,同时间点对照组分别为(271.11±1.12)ng·L-1、(345.18±3.27)ng·L-1、(380.33±5.03)ng·L-1,差异均具有统计学意义(均为P<0.05),相应时间点实验组显著高于对照组。实验组术后1周、2周、3周、4周VEGF浓度的差异均有统计学意义(均为P<0.05),各时间点与术前(128.75±3.83)ng·L-1差异均有统计学意义(均为P<0.05)。对照组术后各时间点VEGF浓度与术前差异均有统计学意义(均为P<0.05)。结论血管内皮细胞移植到角膜内表面后,房水VEGF浓度在术后4周内持续升高,之后稳定在一较高水平。

Objective To explore the changes of vascular endothelial growth factor（VEGF） in aqueous humor after rhesus monkey corneal endothelial cells（CEC） substituted by vascular endothelial cells（VEC）.Methods The rhesus monkey VEC was cultivated to proliferation in vitro,and transplanted onto the posterior surface of rhesus monkey cornea without Descemet membrane by centrifugal sedimentation,the aqueous humor was collected at pre-operation and postoperative 1 week,2 weeks,3 weeks,4 weeks,5 weeks,6 weeks,8 weeks and 12 weeks to measure the concentration of VEGF by ELISA,and then analyzed statistically.Results The concentrations of VEGF in aqueous humor between experimental group and control group showed no significant difference at postoperative 1 week;The concentrations of VEGF at 2 weeks,3 weeks and 4 weeks in experimental group were（374.74±3.30）ng·L-1,（419.06±1.39）ng·L-1 and（481.83±3.36）ng·L-1,which in control group were（271.11±1.12）ng·L-1,（345.18±3.27）ng·L-1 and（380.33±5.03）ng·L-1,there were statistical differences（all P0.05）.There were statistical differences between postoperative 1 week,2 weeks,3 weeks and 4 weeks in experimental group（all P0.05）,and compared with pre-operation（128.75±3.83）ng·L-1,there were statistical differences（all P0.05）.Compared with pre-operation,there were statistical differences at postoperative time points in control group（all P0.05）.Conclusion After CEC are substituted by VEC,VEGF concentration in aqueous humor continues to rise within postoperative 4 weeks,and then stabilize at a high level.