银杏叶提取物增强大鼠视网膜光损伤模型抗氧化应激能力

Ginkgo biloba extract(EGb 761) enhancing retinal anti-oxidation capability after light-induced retinal damage in rats

目的研究银杏叶提取物(extract of Ginkgo biloba,EGb761)对大鼠视网膜光损伤后抗氧化应激状态的影响,探讨EGb761对视网膜光损伤后感光细胞的保护机制。方法 24只雌性SD大鼠随机分为正常对照(NC)组、模型(M)组、模型+生理盐水(MN)组和模型+EGb761(ME)组,每组6只。MN组和ME组分别于造模前1周腹腔注射相应体积的生理盐水和3.5g·L-1EGb761(100mg·kg-1);于光照造模后24h进行神经视网膜组织丙二醛(malondialdehyde,MDA)、总超氧化物歧化酶(total superoxide dismutase,T-SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、过氧化氢酶(catalase,CAT)检测。结果光照24h后M组、MN组和ME组视网膜MDA水平分别为(33.87±1.19)nM·g-1、(31.13±1.50)nM·g-1、(24.33±3.23)nM·g-1,均高于NC组的(20.00±1.99)nM·g-1,差异均有统计学意义(均为P<0.05)。M组和MN组之间MDA水平相近,差异无统计学意义(P>0.05);ME组MDA水平显著低于M组和MN组,差异均有统计学意义(均为P<0.05);M组和MN组T-SOD、GSH-Px、CAT活性均相近,差异均无统计学意义(均为P>0.05),均显著低于NC组和ME组,差异均有统计学意义(均为P<0.05),而ME组T-SOD、GSH-Px、CAT活性均低于NC组,差异均有统计学意义(均为P<0.05)。结论腹腔注射银杏叶提取物EGb761能增强光损伤后视网膜抗氧化应激能力,从而保护视网膜光损伤后光感受器细胞。

Objective To investigate the effect of ginkgo biloba extract（EGb 761） on retinal anti-oxidation capability after light-induced retinal damage（LRD） in rats,and discuss the protective mechanism of EGb 761 on the photoreceptor cells.Methods Twenty-four female Sprague Dawley（SD） rats were randomly divided into normal control group（NC group）,LRD model group（model group）,LRD model ＋ normal saline group（MN group） and LRD model ＋ EGb 761 group（ME group）,6 cases in each group.Rats in MN group and ME were intraperitoneally injected with normal saline and 3.5 g·L-1 EGb 761（100 mg·kg-1）at one week before light exposure.The levels of malondialdehyde（MDA）,total superoxide dismutase（T-SOD）,glutathione peroxidase（GSH-Px）,and catalase（CAT） in the retinal tissues were determined at 24 hours after light exposure.Results At 24 hours after exposure,the MDA levels in the M,MN,ME groups were（33.87±1.19）nM·g-1,（31.13±1.50）nM·g-1 and（24.33±3.23）nM·g-1,significantly higher than that in NC group（20.00±1.99） nM·g-1,there were statistical differences（all P0.05）,those in M group and MN group were similar to each other,there was no statistical difference（P0.05）,and that of ME group was slightly lower than those in M group and MN group,there were statistical differences（all P0.05）.The activities of T-SOD,GSH-Px,and CAT were similar in M group and MN group,there was no statistical difference（all P0.05）,but they were significantly lower than those in NC group and ME group,there were statistical differences（all P0.05）;Their activities in ME group were significantly lower than those in NC group,there were statistical differences（all P0.05）.Conclusion Intraperitoneal injection of EGb 761 can enhance the retinal anti-oxidation capability,thus exert protective effect on photoreceptor cells after LRD.