猪苓及猪苓多糖对BBN诱导的膀胱癌大鼠胸腺、脾指数及CD86表达的影响

Effects of polyporus umbellatus and polyporus polysaccharide on thymus index,spleen index,and CD86 expression in rat bladder cancer

目的观察猪苓及猪苓多糖(PPS)对BBN诱导膀胱癌过程中对大鼠胸腺、脾指数和膀胱组织免疫细胞浸润及CD86表达的影响,从而探讨其抗肿瘤作用中的免疫学机理。方法用N-丁基-N-4-羟丁基亚硝胺(BBN)和糖精水诱导建立Fisher344大鼠膀胱肿瘤模型,实验分6组即对照组、模型组、PPS组(130 mg.kg-1)及猪苓低、中、高剂量组(剂量分别为50、250、500 mg.kg-1)。用称重法测定大鼠的体质量和胸腺、脾脏质量,计算胸腺指数和脾指数;苏木素-伊红(HE)染色观察各组间质淋巴细胞浸润情况,免疫组化法观察各组大鼠膀胱组织CD86的表达情况。结果模型组大鼠胸腺指数显著降低(P<0.05);PPS组的胸腺指数明显高于模型组(P<0.05),猪苓各组脾指数与模型组比较差异无统计学意义。PPS组、猪苓低剂量和中剂量组淋巴细胞浸润较明显,可见淋巴滤泡形成;PPS组与模型组比较有显著性差异(P<0.05)。模型组膀胱肿瘤组织中CD86弱表达或几乎不表达;PPS组膀胱癌上皮CD86表达明显增强,且癌旁上皮CD86有表达。结论猪苓和PPS可通过影响膀胱癌模型大鼠胸腺、脾指数和膀胱组织及癌旁组织淋巴细胞浸润及CD86表达,PPS的作用更显著,猪苓和PPS参与了抑制肿瘤的发生发展过程。

The present study aims to investigate the effects of polyporus umbellatus and polyporus polysaccharide on the index thymus and spleen,the lymphocytic infiltration,and the expression of CD86 in BBN-induced rat bladder cancer.Rats were randomly divided into six groups： control group,BBN group（BBN inducted Fisher344 rat bladder cancer model）,PPS（130 mg·kg-1） group and polyporus（50,250,500 mg·kg-1） groups.Compared with model group,spleen index had no change in PPS group,while the thymus index significantly increased（P 〈 0.05）;H＆E staining illustrated that lymphocyte infiltration was observed obviously in PPS group and polyporus groups（50 and 250 mg·kg-1）,and Folliculi lymphaticus were also observed in these groups;immunohistochemical staining showed that CD86 staining was negative or only weakly positive in model group,while was strong positive in tumor cells of PPS group.These results indicate that polyporus umbellatus and polyporus polysaccharide manifest anti-tumor activity by improving immune function,and the mechanism is presumably due to their effects on thymus and spleen index,lymphocytic infiltration and the expression of CD86.