尿路上皮癌胚抗原1(UCA1)在人膀胱癌组织及膀胱癌细胞株中特异表达

Specific Expression of Urothelial Carcinoma Antigen 1 in Human Bladder Carcinoma Tissues and Cultured Bladder Cancer Cells

UCA1(urothelial carcinoma antigen 1)为自主研发的1个尿路上皮癌基因.应用实时荧光定量PCR检测UCA1 mRNA在2种膀胱癌细胞系、11种非膀胱癌细胞系、18对膀胱癌组织和配对癌旁正常膀胱组织中的表达,对表达差异进行统计学分析.结果显示,UCA1在2种膀胱癌细胞系中显著高表达,而在其他11种非膀胱癌细胞系中表达水平很低或者不表达,二者表达差异达14～24 812倍,差异有统计学意义(P<0.001);在18例膀胱癌组织中,UCA1的平均表达水平是癌旁正常膀胱组织的12.4倍,表达差异有统计学意义(P<0.001).实时荧光定量PCR使UCA1在膀胱癌细胞系及组织中的特异性高表达得以量化.实验结果明确了UCA1作为潜在的肿瘤标记物在膀胱癌临床诊断中的意义,为定量检测尿液UCA1表达并确定诊断膀胱癌的参考值打下基础.

We have previously isolated and indentified a novel gene,referred to as urothelial carcinoma antigen 1（UCA1）.Real-time PCR was used to quantitatively detect UCA1 mRNA in 2 types of bladder cancer cell lines,11 non-bladder cancer cell lines,18 cases of bladder cancer tissues and paired adjacent normal bladder urothelial tissues.The expression difference was statistically analyzed.The results showed that UCA1 expression in the bladder cancer cell lines was significantly increased compared with that in the non-bladder cancer cell lines by 14~24 812 folds（P0.001）.The average UCA1 level was 12.4 fold greater over-expressed in the bladder cancer tissues than that in adjacent normal bladder tissues（P0.001）.The high expression of UCA1 in both bladder cancer cell lines and tissues were quantified by real time PCR,based on which quantitative detection of UCA1 in urine samples will be fulfilled and the reference value range of UCA1 for diagnosis of bladder cancer will be obtained.The results clearly imply UCA1 is a potential tumor biomarker in the diagnosis of bladder cancer and provide a reliable experimental basis for further research.