电子束辐照对透明质酸功能及结构特性的影响

Effect of Electron Beam Irradiation on Structure and Function of Hyaluronic Acid

为了探讨电子束辐照对透明质酸功能及结构特性的影响,选择5、10、20、40、80、100和150kGy的电子束辐照固体透明质酸,测定透明质酸辐照前后分子量、特性粘度、pH值、抗氧化性、紫外光谱、红外光谱、电镜图片的变化.结果表明,辐照降低透明质酸的分子量、特性粘度、pH值;透明质酸在辐照前后的吸收特征峰没有显著的改变,吸收强度增强;透明质酸形状随着辐照剂量的升高,由块状逐渐变成颗粒状;透明质酸对DPPH.自由基的清除作用和还原力随着辐照剂量的增大逐渐增强.电子束辐照对透明质酸分子结构和功能有一定的影响,但对其一级结构没有影响.

To investigate the effects of electron beam irradiation on structure and function of hyaluronic acid（HA）,HA were irradiated at 5.0,10.0,20.0,40.0,80.0,100.0 and 150.0 kGy by electron beam,respectively,to detect the changes of molecular weight,viscosity,pH,oxidation resistance,ultraviolet spectrum,infrared spectrum and electron microscopy images.It turned out that molecular weight,pH and viscosity of HA were reduced after irradiation.There were no significant changes at the characteristic absorption peaks,while there was enhancement in absorption intensity.With the increasing irradiation,the shape of HA gradually were transformed from bulk to granulate and the scavenging ability against DPPH· radicals and increased for the reducing power.Though electron beam irradiation had certain effect on molecular structure and function of HA,there was no effect on its primary structure.