期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

Slit-Robo信号对粘液表皮样癌细胞增殖作用的影响 被引量：3

Role of Slit-Robo signaling in the proliferation of human mucoepidermoid carcinoma Mc3 cells
下载PDF
导出
摘要 目的探讨Slit-Robo信号对粘液表皮样癌细胞系Mc3细胞生长与增殖作用的影响及其作用机制。方法免疫组织化学方法及流式细胞术细胞内因子检测法检测Mc3细胞Slit2及Robo1蛋白的表达;在Robo1受体胞外区的单克隆抗体R5作用下,以细胞计数法与软琼脂克隆形成实验观察Mc3细胞生长变化及用流式细胞术细胞内因子检测法检测增殖相关蛋白PCNA的表达。结果 Mc3细胞表达Slit2及Robo1蛋白,并且在R5单克隆抗体作用下,Mc3细胞体外生长能力受到抑制,增殖能力明显减弱,PCNA蛋白表达水平下降。结论 Slit-Robo信号可能影响Mc3细胞的生长增殖能力,对肿瘤细胞内的PCNA有正调控作用,提示该信号可能参与粘液表皮样癌的发生及发展过程。 Objective To study the role of Slit-Robo signaling in the proliferation of human mucoepidermoid carcinoma Mc3 cells.Methods We measured the expressions of Slit2 and Robo1 proteins in human mucoepidermoid carcinoma Mc3 cells using immunohistochemistry and flow cytometry.To test the role of Slit-Robo signaling in the proliferation of the cells,we treated the cells with the monoclonal antibody R5 against Robo1 receptor extracellular domain and observed the changes in the cell proliferation by cell counting and colonogenic assay;the expression of proliferating cell nuclear antigen（PCNA） in the cells following the treatment was measured with flow cytometry.Results Treatment of Mc3 cells with the monoclonal antibody R5 caused significantly suppressed cell growth and proliferation and obviously lowered the expression of PCNA.Conclusion Slit-Robo signaling can suppress the proliferation of Mc3 cells possibly by up-regulating the expression of PCNA.
作者 韩冰 王丽京 王静 张洁 赵媛 亓翠玲
机构地区 西北民族大学医学院 广东药学院血管生物学研究所 兰州大学口腔医学院
出处 《南方医科大学学报》 CAS CSCD 北大核心 2012年第1期37-39,45,共4页 Journal of Southern Medical University
基金 国家自然科学基金(30871304) 国家科技部"973"项目(2010CB529702) 中央高校基本科研业务费专项资金(lzujbky-2010-196)~~
关键词 粘液表皮样癌 Slit-Robo信号 Mc3细胞 R5mAb 增殖细胞核抗原 mucoepidermoid carcinoma Slit-Robo signaling Mc3 cells R5 monoclonal antibody proliferating cell nuclear antigen
分类号 R739.8 [医药卫生—肿瘤]
  • 相关文献

参考文献11

  • 1Li HS, Chen JH, Wu W, et al. Vertebrate slit, a secreted ligand for the transmembrane protein roundabout, is a repellent for olfactory bulb axons[J]. Cell, 1999, 96(6): 807-18.
  • 2Wu JY, Feng L, Park HT, et al. The neuronal repellent Slit inhibits leukocyte chemotaxis induced by chemotaetic factors [J]. Nature, 2001, 410(6831): 948-52.
  • 3Wang B, Xiao Y, Ding BB, et al. Induction of tumor angiogenesis by Slit-Robo signaling and inhibition of cancer growth by blocking Robo activity[J]. Cancer Cell, 2003, 4(1): 19-29.
  • 4Kaur S, Castellone MD, Bedell VM, et al. Robo4 signaling in endothelial ceils implies attraction guidance mechanisms[J].J Biol Chem, 2006, 281(16): 11347-56.
  • 5Yiin JJ, Hu B, Jarzynka MJ, et al. Slit2 inhibits glioma cell invasion in the brain by suppression of Cdc42 activity [J]. J Neurooncol,2009, 11(6): 779-89.
  • 6Nasarre P, Potiron V, Drabkin H, et al. Guidance molecules in lung cancer[J]. J Cell Adhesion Migration, 2009, 4(1): 130-45.
  • 7Ito H, Ftmahashi S, Yamauchi N, et al. Identification of ROBO 1 as a novel hepatocellular carcinoma antigen and a potential therapeutic and diagnostic target[J]. Clin Cancer Res, 2006, 12(11 Pt 1): 3257-64.
  • 8Werbowetski-Ogilvie TE, Seyed Sadr M, Jabado N, et al. Inhibition of medulloblastoma cell invasion by Slit [J]. Oncogene, 2006, 25 (37): 5103-12.
  • 9Xian J, Clark K J, Fordham R, et al. Inadequate lung development and bronchial hyperplasia in mice with a targeted deletion in the Duttl/Robol gene[J]. Proc Natl Acad Sci USA, 2001, 98(26): 15062-6.
  • 10Xian J, Aitchison A, Bobrow L, et al. Targeted disruption of the 3p12 gene, Duttl/Robol, predisposes mice to lung adenocarcinomas and lymphomas with methylation of the gene promoter [J]. Cancer Res, 2004, 64(18): 6432-7.

同被引文献29

  • 1王忠富,侯毅斌,李建生,卢斌贵,陈国硕,汤日杰.78例腮腺肿瘤的CT图像特点及诊断价值[J].医学信息（医学与计算机应用）,2014,0(29):37-38. 被引量：1
  • 2Li H S,Chen J H.Wu W,et al. Vertebrate slit,a secreted lig-and for the transmembrane protein roundabout,is a repellentfor olfactory bulb axons[J]. Cell.1999,96(6) :807-818.
  • 3Wu J Y,Feng L,Park H T,et al. The neuronal repellent Slitinhibits leukocyte chemotaxis induced by chemotactic factors[J]. Nature,2001,410(6831) :948-952.
  • 4Gu X’Pan H,Liang Y,et al. Implementation and evaluation ofvarious demons deformable image registration algorithms on aGPU[J]. Phys Med Biol,2010,55(1) :207-219.
  • 5Vercauteren T,Pennec X,Perchant A,et al. Diffeomorphic de-mons: efficient non-parametric image registration[J]. Neuro-image,2009,45(Suppl 1):S61-S72.
  • 6Yiin J J . Hu B, Jarzynka M J, et al. Slit2 inhibits glioma cellinvasion in the brain by suppression of Cdc42 activity [J].Neuro Oncol,2009,11(6) :779-789.
  • 7Nasarre P’Potiron V’Drabkin H,et al. Guidance molecules inlung cancer[J]. Cell Adh Migr,2010 ,4(1) : 130-145.
  • 8Ito H, Funahashi S,Yamauchi N, et al. Identification of RO-BOl as a novel hepatocellular carcinoma antigen and a poten-tial therapeutic and diagnostic target [J] . Clin Cancer Res,2006,12(11 Pt 1)-3257-3264.
  • 9Ellis J. Silencing and variegation of gammaretrovirus and le-ntivirus vectors [J] . Hum Gene Ther,2005,16 ( 11 ) : 1241-1246.
  • 10Dissen G A,Lomniczi A,Neff T L,et al. In vivo manipulationof gene expression in non-human primates using lentiviralvectors as delivery vehicles[J]. Methods,2009,49(1) :70-77.

引证文献3

二级引证文献11

南方医科大学学报
2012年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部