IL-17对肺成纤维细胞的增殖、转化和胶原合成作用

Effects of interleukin-17 on murine pulmonary fibroblast proliferation,transformation and collagen synthesis

目的探讨IL-17对博来霉素诱导肺纤维化形成中的肺成纤维细胞增殖、转化、胶原合成作用。方法用5 mg/kg博来霉素气管内注入的方法诱导肺纤维化形成,逆转录-聚合酶链反应(RT-PCR)检测IL-17/IL-17RmRNA在肺纤维化形成中表达的变化,选择表达IL-17RmRNA最佳时间的肺成纤维细胞进行原代培养、鉴定。使用四氮唑蓝比色法检测不同浓度的IL-17对肺成纤维细胞的增殖。使用RT-PCR和Western Blotting检测肌成纤维细胞标志物α-SMA以及I和III型胶原的表达。结果(1)IL-17/IL-17RmRNA在肺纤维化形成中明显升高,在第14天表达最高。(2)不同浓度IL-17能够促进肺成纤维细胞的增殖,最佳浓度为50 ng/ml。(3)IL-17促进肺成纤维细胞表达α-SMA增加。(4)IL-17促进肺成纤维细胞I和III型胶原合成。结论 IL-17具有促进小鼠肺纤维化形成中肺成纤维细胞增殖、转化和胶原的合成作用,可能在肺纤维化形成中起重要作用。

Objective To investigate the effects of interleukin-17（IL-17） on the proliferation,transformation and collagen synthesis of the lung fibroblasts in mice with bleomycin-induced pulmonary fibrosis.Methods In a mouse model of pulmonary fibrosis established by intratracheal administration of 5 mg/kg bleomycin,the dynamic expressions of IL-17/IL-17 receptor（IL-17R） mRNAs were detected by RT-PCR.At 14 days following bleomycin administration,the pulmonary fibroblasts were isolated,cultured and identified.MTT assay was used to assess the proliferation of the pulmonary fibroblasts in response to IL-17 treatment at different concentrations,and RT-PCR and Western blotting were employed to examine the mRNA and protein expressions of α-smooth muscle actin（α-SMA） and types I and III collagen.Results IL-17/IL-17R mRNA levels were increased obviously in the pulmonary fibroblasts of rats with pulmonary fibrosis,and the highest expressions occurred at 14 days following bleomycin administration.Exogenous IL-17,at the optimal concentration of 50 ng/ml,significantly promoted the proliferation of the pulmonary fibroblasts in primary culture and obviously increased α-SMA expression and types I and III collagen synthesis in the fibroblasts.Conclusion IL-17 can promote the proliferation,transformation,and collagen synthesis of the pulmonary fibroblasts from rats with bleomycin-induced pulmonary fibrosis.