铃兰组织培养及快速繁殖的研究

The Study of the Tissue Culture and Rapid Propagation of Convallaria Keiskei Miq

为保存野生资源,满足栽培对种苗的需要,以根茎为材料,采用组织培养的方法,进行了根茎的分化、分化芽生根及快速繁殖、试管苗的移栽和定植的研究。结果表明:MS+6-BA 0.3 mg/L+NAA 0.1 mg/L是根茎芽生长与分化的理想培养基;White+NAA 0.1 mg/L+IAA 0.2 mg/L是根茎生长分化芽生长生根培养的理想培养基。延长生根培养时间,向培养瓶中加入液体培养基使根茎分化的方法,达到了快速繁殖的目的。由根茎繁殖的试管苗定植后,保持了野生铃兰的所有生物性状。

In order to preserve the wild resources and meet the needs of germchits for planting,with method of tissue culture,root stems of Convallaria keiskei Miq were used as material to do the research on differentiation of root stems,rooting and rapid propagation of adventitious buds,transplantation of tube seedlings.The results show that MS＋6-BA 0.3 mg/L＋NAA 0.1 mg/Lwas suitable for growth and differentiation of root stems;the best medium for rooting of adventitious buds was White＋NAA 0.1 mg/L＋IAA 0.2 mg/L.The method of extend the time for rooting and add liquid medium to the culture flask for differentiation achieve the purpose of rapid propagation.The test tube seedlings from root stems maintained all biological characteristics of the wild Convallaria keiskei after transplanted.