刺参池塘底栖真核生物DGGE指纹结构与环境理化因子的相关性分析

DNA fingerprinting structure of benthic eukaryotic structure and its relations to environmental physical and chemical factors in sea cucumber culture ponds

采用基于18SrDNA PCR-DGGE技术对刺参池塘养殖系统底栖真核生物群落结构与环境理化因子进行分析。结果表明,刺参养殖池塘系统中附着基真核生物DNA指纹图谱均呈现出了丰富的多样性,成参养殖池塘分别获得26、25、25条扩增条带,幼参养殖池塘分别获得20、22条扩增条带;刺参养殖池塘中叶绿素、总磷、总氮、溶氧分别为2.037～5.383μg/L、0.016～0.039mg/L、1.863～3.562mg/L、4.06～6.18mg/L,成参养殖池塘的各项理化指标值均高于幼参养殖池塘。通过指纹图谱和理化因子的聚类分析可以看出,附着基底栖真核生物DGGE指纹图谱与环境中理化因子聚类分析图相吻合,说明不同刺参养殖池塘底栖真核生物群落DNA指纹与养殖环境的理化因子密切相关。本研究通过DGGE技术分析刺参池塘附着基底栖真核生物指纹图谱,进而分析与环境中理化因子的关系,为建立底栖真核生物群落DNA指纹结构与水体理化指标之间的关系提供科学依据。

DNA fingerprinting structure of benthic eukaryotic community and its relations to environmental physical and chemical factors in sea cucumber culture ponds were studied. The DNA fingerprinting structure was measured using PCR-DGGE technique and the physical and chemical factors of sea cucumber ponds were analyzed with general methods. Then the relation- ship were assessed with cluster analysis. The number of amplified DGGE bands for adult sea cucumber in three culture ponds and juveniles in two ponds were 26, 25, 25 and 20, 20 respectively, which indicated that the ponds culturing adult sea cucumber had richer eukaryotic-diversity. Physical and chemical factors including chlorophyll content, total phosphorus, total nitrogen and dissolved oxygen in sea cucumber culture ponds were 2. 037- 5. 383μg/L, 0. 016 -0. 039 mg/L, 1. 863-3. 562 mg/L, 4.06-6.18 mg/L, respectively. All of the physical and chemical factor values in ponds culturing adult sea cucumber were higher than that of ponds culturing juvenile sea cucumber. The result of cluster analysis of environmental physical and chemical factors was similar with that of the benthic eukaryotic community based on PCR-DGGE, which suggested that the benthic eukaryotic community was closely associated with the physical and chemical factors in sea cucumber ponds.