高效降解废弃蓖麻基润滑油降解菌的分离筛选及特性研究

Isolation of Castor Oil-based Waste Degrading Bacteria and Its Degradation Characteristics

从内蒙古某蓖麻榨油厂排污口采样,分离筛选出10株能降解废弃蓖麻基润滑油菌株,其中T-9菌株降解润滑油的能力较强,该菌株最适降解pH值为5.0,降解温度30℃,在1%～5%的NaCl中能较好生长.通过菌落形态与生理生化实验,初步鉴定该菌株为假单胞菌属(Pseudomonas).在润滑油质量浓度为10 g/L,初始pH值为5.0,180 r/min,30℃下培养7 d后,采用改进的CEC-L-33-A-93方法测得其对废弃蓖麻基润滑油的降解率为72%.采用GC/MS对降解产物进行分析,测得其对废弃蓖麻基润滑油降解率为80%,该菌株具有良好的蓖麻基润滑油降解能力.

Ten strains which can degrade the waste lubricants were isolated from sewage samples of the outfall of a castor oil extraction plant in Inner Mongolia,China,and T-9 strain showed the strongest lubricant degradation ability.The optimal degradation condition of T-9 strain was pH 5.0,and temperature 30 ℃.T-9 strain could grow well in the medium containing 1%~5% NaCl.T-9 strain was identified as Pseudomonas by the colony morphology,physiological and biochemical characteristics.By improved CEC-L-33-A-93 method,the degradation rate of castor oil-based waste lubricants was 72% when incubated for 7 days under the conditions as following： 10 g/L oil,initial pH 5.0,rotation speed of 180 r/min,and 30 ℃.By GC/MS analysis of degradation products,degradation rate was 80%,indicating that the T-9 strain had good castor-based oil degradation ability.