鸡精中呈味核苷酸二钠的高效液相色谱法测定

Quantitative Determination of Disodium 5′-Inosinate and Disodium 5′-Guanylate in Chicken Extract by HPLC

建立了高效液相色谱法测定呈味核苷酸二钠(IMP和GMP)含量的方法.以四丁基磷酸铵和磷酸二氢钾配以一定比例的乙腈为流动相,采用高耐水系Waters RP 18色谱柱,通过色谱条件的优化,获得了定性定量重复性相对标准偏差<2%的标准品色谱图,其中,IMP标准曲线在60～600μg/mL范围内的线性相关系数为R=1.0000,GMP标准曲线在50～480μg/mL范围内的线性相关系数为R=0.999 9.测得某品牌的鸡精内IMP质量含量为0.54%,GMP质量含量为0.60%,两次测定结果的精密度GMP为0.73%,IMP为1.17%.

A high performance liquid chromatography method was established for quantitative determination of disodium 5′-inosinate and disodium 5′-guanylate（IMP and GMP）.Using tetrabutylammonium phosphate monobasic and potassim dihydrogen phosphate mixed with a certain proportion of acetonitrile as the mobile phase,the standard samples were analyzed on Waters RP 18 column under the optimal conditions.The qualitative and quantitative reproducibility of RSD were below 2%.On the IMP standard curve,the linear range was 60~600 μg/mL,the correlation coefficient R being 1.000.On the GMP standard curve,the linear range was 50~480 μg/mL,the correlation coefficient R being 0.999 9.The method was used to determine IMP and GMP content in a certain brand of chicken extract,which was 0.54% and 0.60%,respectively,the precision being 0.73% for GMP,1.17% for IMP.