环氧啉含量的HPLC法测定方法

Determination of cycloxylidin by HPLC

建立了环氧啉的高效液相色谱(HPLC)的测定方法,在C18色谱柱(C18250mm×4.6mm,5μg/mL)上,以甲醇-水(25∶75,V/V)为流动相,在流速为1.0mL/min,室温条件下,220nm波长检测,环氧啉标准样品分离效果良好.环氧啉溶液在43.2～345.6μg/mL范围内浓度与峰面积线性关系良好,相关系数r=0.999 92;方法的检测限为0.4μg.该方法简单快速,准确,可靠,为环氧啉的工业生产质量控制提供一定的保证.

The method for the determination of Cycloxylidin by high performance liquid chromatography （HPLC） was established. It used C18 column （250 mm × 4.6 mm, 5 ug/mL） to separate the sample, 25% methanol and 75% water as the mobile phase,and the Cycloxylidin was detected at 220 nm with the flow rate of 1. 0 mL/min at ambient temperature. The result showed that the linear relative coefficient was 0. 999 92 while in a range of 43.2--345. 6 ug/mL; the limit of this method was 0.4 ug. This method was simple,rapid and effective in the quality control for production of Cycloxylidin.